几种野牡丹属植物系统发育关系的初步研究  

Compare Study on Phylogenetic Relationships among Several Species in Melastoma ( Melastomaceae)

作　　者：代色平[1] 刘连海[1] 吴伟[2] 

机构地区：[1]广州市园林科学研究所,广东广州510405 [2]中国科学院华南植物园,广东广州510605

出　　处：《广东林业科技》2013年第4期7-10,共4页Forestry Science and Technology of Guangdong Province

基　　金：广东省科技项目(2008B021500005);广东省科技项目(2009B050700046);广州市条件建设项目(2060502);广东省热带亚热带植物资源重点实验室开放课题(plant01k04)

摘　　要：采用nr ITS和叶绿体基因间隔区trnL-trnF的序列,初步研究分布于广东省的野牡丹属的6个物种,即展毛野牡丹(Melastoma normale)、野牡丹(M.candidum)、多花野牡丹(M.affine)、细叶野牡丹(M.in-termedium)、地稔(M.dodecandrum)、毛稔(M.sanguineum)和1个原产印度的印度野牡丹(M.malabathri-cum)及其白花变种(M.malabathricum var.alba)的遗传关系。结果表明,展毛野牡丹、野牡丹、多花野牡丹、细叶野牡丹和毛稔这5个物种具有完全一样的nr ITS和叶绿体基因间隔区trnL-trnF序列,表明它们之间存在非常近的亲缘关系。印度野牡丹在nr ITS序列上与前5个物种序列相同,但在叶绿体trnL-trnF序列上具有1个碱基的差异,表明它与这些物种之间存在非常近缘的关系。地稔与其他几个物种无论在nr ITS序列还是在叶绿体trnL-trnF序列上都差异甚大。In this study, nuclear ribosomal internal transcribed spacer （nr ITS）and chloroplast trnL-trnF sequences were used to clarify the relationships of seven species in Melastoma, which include six species from Chi- na and one species from India. The results showed that M. normale, M. candidum, M. affine, M. intermedium and M. sanguineum had identical nr ITS and chloroplast trnL-trnF sequences, suggesting their close relationships. M. malabathricum and its variety possessed identical nr ITS sequences with the five species, but differed from them by one nueleotide substitution in chloroplast trnL-trnF sequences, implying close relationships among them. M. do- decandrum was substantially different from other species in both nr ITS and chloroplast trnL-trnF sequences.

关 键 词：野牡丹属 遗传关系 NR ITS序列 叶绿体trnL-trnF序列 

分 类 号：S718.46[农业科学—林学]