小鼠巨噬细胞吞噬功能测定方法的改进及应用(英文)  

Improvement and Application of Assay for Quantitating Phagocytosis of Mice Peritoneal Macrophages

作　　者：余虹[1] 

机构地区：[1]重庆医药高等专科学校药学院,中国重庆400030

出　　处：《生命科学研究》2013年第4期316-320,共5页Life Science Research

基　　金：重庆医药高等专科学校自然科学基金资助项目(2010108)~~

摘　　要：对巨噬细胞吞噬鸡红细胞活性的体内检测方法进行改进.收集小鼠腹腔巨噬细胞,加入不同浓度甘草多糖、鸡红细胞于37℃共同孵育1 h,在倒置显微镜下观察吞噬状态,统计吞噬百分率和吞噬指数.并将改进后的体外吞噬方法用于检测酵母多糖对小鼠腹腔巨噬细胞形态和吞噬功能的影响.结果表明:改进后的体外吞噬法测定的结果与传统的体内吞噬法比较,两者具有显著的相关性(n=6,P<0.01).酵母多糖对巨噬细胞刺激1 h后,与对照组相比,其吞噬功能显著性增强;巨噬细胞形态上出现被活化的特征.应用改进后的方法研究巨噬细胞吞噬鸡红细胞,不需染色,在模拟生理条件下进行,保持了细胞活性,有简便、成本低以及准确率高的特点,适用于普通实验室的科研和教学.The traditional in vivo assay for quantitating phagocytosis of murine peritoneal macrophages was improved. The macrophages were collected from the peritoneal cavity of BALB/C mice. The macrophages treated with glycyrrhiza polysaccharide at different concentrations were incubated with chicken red blood cells （CRBC） at 37℃ with 5% CO2 in the air. After 1 h, the phagocytic percentages and index were calculated under inverted microscope. Meanwhile, the improved method was utilized to evaluate the effects of zymosan on the phagocytosis and morphology of macrophages. The results indicated that the phagocytic percentages and index by the improved assay were significantly correlated with that of in vivo assay （n=6, P〈0.01）. After 1 h of zymosan treatment, the phagocytosis was significantly enhanced by zymosan compared with con- trol; obvious morphological changes of macrophages were observed, those typical morphological features sug- gested that macrophages might have been activated. The new improved in vitro assay for quantitating phagocytosis without staining was performed under the simulated physiological conditions, which has been keeping the cell activities. It is an easy, fast, cost efficient and reliable method suitable for scientific research and teaching application in a routine laboratory.

关 键 词：巨噬细胞 鸡红细胞 吞噬 体外 

分 类 号：Q331[生物学—遗传学]