头花蓼愈伤组织诱导及分化  

Callus Induction and Differentiation of Polygonum capitatum Buch

作　　者：郭彪[1] 李雪松[1] 李政[1] 杨静慧[1] 李凌[1] 

机构地区：[1]西南大学园艺园林学院,重庆400716

出　　处：《安徽农学通报》2013年第17期21-23,55,共4页Anhui Agricultural Science Bulletin

摘　　要：研究了2种外植体(叶片、茎段)、消毒方法、培养基种类、活性炭、植物生长调节剂等因素对头花蓼愈伤组织诱导及分化的影响。结果表明,最佳消毒方法为以0.15%HgCl2消毒叶片6min和10%NaClO消毒茎段9min;不同培养基诱导愈伤组织发生率依次为MS>1/2MS>B5;MS+6-BA 1.0mg/L+NAA 0.3mg/L有利于愈伤组织形成;MS+6-BA 4mg/L+NAA 0.2mg/L有利于愈伤组织增殖;MS+6-BA 8mg/L+IBA 0.1mg/L有利分化,分化率为83.33%,产生不定芽平均数为5.1个。培养基中附加100mg/L活性炭,褐化率仅为6.80%,愈伤组织生长和分化效果较好。Effects of two kinds of explant types（leaf, stem-segment）,disinfection method, medium type, activated car- bon and different plant growth regulators on inducing calls of P.capitatum were studied.The resuh showed that the best effects of disinfection method,the leaf was treatedapp：addword：treated by 0.15%HGC12 for six minutes and the stem-segment was treated byl0% NaC10 for nine minutes.The order of calls engendering proportion in different mediums was MS〉I/2MS〉BS. The optimum medium for callus formation,proliferation and differentiation were MS＋6-BA 1.0mg/L＋NAA 0.3mg/L,MS＋6-BA 4.0mg/L＋NAA 0.2mg/L and MS＋6-BA 8mg/L＋IBA 0.1mg/L. The differentiation rate was 83.33% and resulting thetdventitious buds was 5.1 in an average.Medium supple- mented with activated carbon 100mg/L,the browning rate was 6.80%, and callus growth better.

关 键 词：头花蓼 叶片 茎段 愈伤组织 

分 类 号：S58[农业科学—作物学]