利用染色体片段置换系定位水稻抗纹枯病QTLs  

Mapping of QTLs for sheath blight resistance using chromosome segment substitution lines of rice (Oryza sativa L.)

作　　者：林静[1] 张所兵[1] 张云辉[1] 汪迎节[1] 方先文[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省种质资源保护与利用研究中心,江苏省优质水稻工程技术研究中心,国家水稻改良中心南京分中心,江苏南京210014

出　　处：《江苏农业学报》2013年第4期691-695,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(11)1020]

摘　　要：为检测水稻纹枯病抗性基因位点,本研究采用牙签接种法对籼稻9311 和粳稻日本晴以及由它们构建的119 个染色体片段置换系进行纹枯病抗性鉴定,并使用该群体的分子连锁图谱进行数量性状座位（QTL）分析。共检测到3 个纹枯病相关QTLs（qsb8-1、qsb8-2 和qsb8-3）,分别位于第8 染色体相邻标记RM3262、RM5485 和RM3496 附近,所在遗传区间分别为81. 7 -91.7 cM、91. 7-108.1 cM 和108. 1-119.6 cM。qsb8-2 的加性效应为负值,表明感病亲本携带的该片段可以增强纹枯病抗性;qsb8-1 和qsb8-3 的加性效应为正值,表明感病亲本携带的该片段减弱了纹枯病抗性。and Japonica Nipponbare was employed to map quantitative trait loci （QTL） for sheath blight resistance in rice base on the toothpick method. Three sheath bilght resistant QTLs（qsb8-1,qsb8-2 和qsb8-3）were identified, which were located on connected molecular markers RM3262, RM5485 and RM3496 of chromosome 8. The genetic interval were 81.7-91.7 cM, 91.7-108.1 cM and 108.1-119.6 cM, respectively. Additive effect of qsb8-2 was negative, indicating that sus-ceptible parent carrying the fragment can enhance resistance to sheath blight. However, additive effects of qsb8-1 and qsb8-3 were positive, indicating that susceptible parent carrying the fragment weakened resistance to sheath blight.

关 键 词：水稻 纹枯病抗性 染色体片段置换系 数量性状基因定位 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治]