花生种子脂肪氧化酶的分离纯化和鉴定  

Purification and identification of lipoxygenase of peanut seed

作　　者：张琛[1] 李泽福[2] 滕斌[2] 汪顺丽[1] 高奇[1] 张瑛[2] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽省农业科学院水稻研究所/安徽省水稻遗传育种重点试验室,安徽合肥230031

出　　处：《中国油料作物学报》2013年第4期411-414,共4页Chinese Journal of Oil Crop Sciences

基　　金：安徽省自然科学基金(11040606M97);安徽省水稻遗传育种重点实验室开放基金(SD2009-4)

摘　　要：利用硫酸铵盐析分级分离、DEAE-52离子交换层析及葡聚糖凝胶层析(Sephadex G-150)过滤,从花生种子中获得了4种花生脂肪氧化酶同工酶(B-II、B-III和C-V、C-VI),所得纯化产物经SDS-PAGE检验为均一单带,4种花生脂肪氧化酶同工酶均由一种多肽链组成,其亚基相对分子质量约为31kD,其纯化倍数为63.2,50.7,22和55.45,产率分别为4.8%,5.3%,2%和10%。Four isoenzymes of peanut lipoxygenases,B-III,B-III,C-V and C-VI were isolated from peanut seed by using ammonium sulfate precipitation,DEAE-52 ion exchange chromatography and Superdex G-150 gel filtration.These lipoxygenases were comprised of one equal subunit in SDS-PAGE experiment,and relative molecular mass of this subunit was 31kD.The final purification fold of B-II,B-III,C-V and C-VI were 63.2,50.7,22 and 55.45 respectively,and their yield of LOX were 4.8%,5.3%,2% and 10% respectively.

关 键 词：花生 脂肪氧化酶 分离纯化 鉴定 

分 类 号：S565.209.9[农业科学—作物学]