Ionic Liquid-based Microwave-assisted Liquid-liquid Microextraction and High Performance Liquid Chromatography Determination of Sulfonamides from Animal Oils  

作　　者：FENG Xu-dong LIANG Fang-hui SU Rui WU Li-jie LI Xue-yuan WANG Xing-hua ZHANG Han-qi YU Ai-min 

机构地区：[1]College of Chemistry, Jilin University, Changehun 130012, P. R. China [2]Department of Pharmacy, Changchun Medical College, Changchun 130117, P. R. China

出　　处：《Chemical Research in Chinese Universities》2013年第4期647-652,共6页高等学校化学研究（英文版）

摘　　要：The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.

关 键 词：Ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） High perfor-mance liquid chromatography（HPLC） Sulfonamide Animal oil 

分 类 号：O657.72[理学—分析化学] O657.71[理学—化学]