抗猫泛白细胞减少症病毒单克隆抗体的制备及其生物学特性鉴定  

Development of Monoclonal Antibody Specific for Feline Panleukopenia Virus and Its Identification of Biological Characteristics

作　　者：钱建飞[1] 陈溥言[2] 蔡宝祥[2] 张振兴[2] 凌雯[1] 李银[1] 

机构地区：[1]江苏省农业科学院畜牧兽医研究所,江苏南京210014 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《中国兽医学报》2000年第4期339-342,共4页Chinese Journal of Veterinary Science

摘　　要：用 PEG60 0 0沉淀和蔗糖密度梯度离心从细胞培养物中纯化猫泛白细胞减少症病毒 ( FPV) ,以纯化FPV免疫 BALB/c小鼠 ,运用淋巴细胞杂交瘤技术 ,获得了 4株抗 FPV的特异性单克隆抗体 ( Mc Ab)。其腹水 Mc Ab的 ELISA效价在 1 0 - 4～ 1 0 - 5之间 ,其中 1株具有血凝抑制能力。经 ELISA阻断试验及 ELISA交叉反应性试验测定 ,这 4株 Mc Ab可与 FPV、犬细小病毒 ( CPV)和水貂肠炎病毒 ( MEV)呈特异性反应 ,因此可作为检测 FPV、CPV和Four monoclonal antibodies(McAbs) specific for feline panleukopenia virus(FPV) were obtained by fusion of SP2/0 myeloma cells with splenocytes from BALB/c mice immunized with purified FPV using the method of polyethyleneglycol 6000 precipitation and density gradient centrifugation from infected cell culture. The ELISA titers of these McAbs were between 10 -4 and 10 -5 . One of them, 3A 9 was able to inhibit the hemagglutination of FPV in hemagglutination inhibition (HI) test. All McAbs specifically reacted with FPV, canine parvovirus(CPV) and mink enteritis virus (MEV) by ELISA blocking test and ELISA cross reactivity test. It is suggested that these McAbs are able to detect the same antigen of FPV, CPV and MEV as a common biochemical.

关 键 词：猫泛白细胞减少症病毒 单克隆抗体 生物学特性 

分 类 号：S858.293[农业科学—临床兽医学] S852.43[农业科学—兽医学]