HPLC同时测定苦参药材中5种生物碱含量  

Determination of Five Kinds of Alkaloids in Sophorae Flavescentis Radix by HPLC

作　　者：吕佳[1] 王丹[1] 张振秋[1] 袁子民[1] 刘玉强[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中国中医药信息杂志》2013年第9期61-62,81,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：大连市科学技术基金(2008J21JH007)

摘　　要：目的使用高效液相色谱法测定不同产地苦参药材中槐果碱、苦参碱、氧化槐果碱、槐定碱、氧化苦参碱的含量。方法使用Agilent ZORBAX NH2(4.6 mm×150 mm,5μm)色谱柱,流动相为乙腈-无水乙醇-3%磷酸溶液(84∶10∶6),流速为1 mL/min,检测波长为210 nm。结果槐果碱在0.02288～0.1144μg(r=0.9997)、苦参碱在0.083 2～0.4160μg(r=0.9997)、氧化槐果碱在0.3762～1.8360μg(r=0.9998)、槐定碱在0.1044～0.522μg(r=0.999 2)、氧化苦参碱在0.491 2～2.456μg(r=0.999 9)范围呈良好的线性关系,加样回收率分别为101.63%(RSD=2.08%)、98.29%(RSD=1.87%)、101.89%(RSD=1.97%)、99.87%(RSD=2.06%)、102.66%(RSD=1.34%)。结论该方法简便、灵敏、准确,可用于苦参药材的质量控制。Objective To determine the content of sophocarpine, mat_fine, oxysophocarpine, sophoridine, oxymatrine in Sophora Flavescentis Radix from different areas. Methods Agilent ZORBAX NH2 column （4.6 mmX 150 mm, 5 lain） was used with mobile phase of acetonitrile-ethanol-3% phosphate （84 ： 10 ： 6）, at a flow rate of 1 mL/min. The wavelength of detection was 210 rum. Results The Linear range of sophocarpine, rnatrine, oxysophocarpine, sophoridine and oxymatrine were 0.022 88-0.114 4 lag （r= 0.999 7）, 0.083 2-0.416 0 lag （r=0.999 7）, 0.376 2-1.836 0 lag （r=0.999 8）, 0.104 4-0.522 lag （r=0.999 2）, 0.491 2-2.456 lag （r=0.999 9）, respectively. The average recovery were 101.63% （RSD=2.08%）, 98.29% （RSD=l.87%J, 101.89% （RSD=1.97%）, 99.87% （RSD = 2.06%）, 102.66% （RSD=1.34%）, respectively. Conclusion The method is simple, rapid and accurate, and suitable for the quality control of Sophorae Flavescentis Radix.

关 键 词：苦参 含量测定 槐果碱 苦参碱 氧化槐果碱 槐定碱 氧化苦参碱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]