小腔游仆虫(Euplotes aediculatus)皮层微管胞器的荧光标记  

Microtubular organelles in Euplotes aediculatus(Ciliophora:Hypotrichida) revealed by fluorescent labeling

作　　者：林钦[1] 范鑫鹏[1] 孙磊[1] 周素娟[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2013年第5期53-60,共8页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(31172042)

摘　　要：应用荧光紫杉醇直接荧光标记和抗a-微管蛋白抗体免疫荧光标记,显示了纤毛虫小腔游仆虫(Euplotes aediculatus)皮层纤毛器微管、纤毛器基部附属微管和背皮层表面微管等结构,以及皮层纤毛器微管的形态发生.结果表明,该游仆虫背皮层表面网在形态上与嗜银网相一致,也是一种微管类细胞骨架;与其他几种游仆虫比较,前仔虫口纤毛器和背皮层纤毛器微管的形态发生存在不同的模式,其形态发生可能具有种的特异性,可以作为种的分类依据之一.此外,与FLUTAX(fluorescence taxoid)标记方法相比较,抗α-微管蛋白抗体免疫荧光标记能较清晰地显示游仆虫背皮层表面微管网及早期发生的额腹横棘毛原基和背触毛原基,推测该游仆虫的细胞背表面微管网与其他微管结构其微管蛋白组分可能存在差异,且形态发生中伴随着皮层纤毛器微管的组装和成熟,其微管蛋白组分同时也在不断发生变化.The cortical ciliature microtubules,base-associated microtubules,the dorsal cortical microtubule structure,as well as the morphogenesis of the cortical ciliature microtubules in Euplotes aediculatus were analyzed with FLUTAX（fluorescence taxoid） and anti-a-tubulin antibody immuno-fluorescence labeling.The results indicate that the dorsal cortical network which is called silver-line system morphologically associates with microtubular cytoskeleton.Moreover, differing from other Euplotes,The morphogenesis mode of the adoral ciliature microtubules and dorsal cortex ciliature microtubules in the proter of E.aediculatus represent a distinct morphogenesis mode which might be a referable basis for species differentiation.Additionally,anti-crtu- bulin antibody immuno-fluorescence labeling shows dorsal cortex network and the early frontalventral -transverse cirri primordia more clearly comparing with fluorescently labeling by FLUTAX.This might suggest the tubulin element is different between cell dorsal cortex surface network and other microtubular structures,and the tubulin element may change gradually as the assembling and maturing of cortical ciliature microtubule in sequential morphogenesis stages.

关 键 词：纤毛虫 小腔游仆虫 荧光标记 皮层纤毛器微管 背皮层表面网 

分 类 号：Q952[生物学—动物学]