PC交联脱细胞牛心包膜的力学性能、稳定性和血液相容性  

Crosslinking of decellularized bovine pericardium matrix by procyanidins:mechanical properties,stability and hemo-compatibility

作　　者：马兵[1,2] 翟万银[2] 张嘉敏[3] 孙晓宁[4] 朱自严[3] 张红锋[1] 常江[2] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]中国科学院上海硅酸盐研究所生物材料与组织工程中心,上海200050 [3]上海市血液中心上海市输血研究所,上海200051 [4]复旦大学附属中山医院心脏外科,上海200032

出　　处：《华东师范大学学报（自然科学版）》2013年第5期61-70,79,共11页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(31070870)

摘　　要：测定了原花青素(PC)交联的脱细胞牛心包膜(dbpECM)的力学性能、稳定性和血液相容性.实验结果显示,2.5 mg/mL原花青素交联能将拉伸强度从29.1 MPa提高到51.3 MPa,并保持弹性模量基本不变,而抗胶原酶降解能力与戊二醛相当;PC-dbpECM浸泡于D-Hanks溶液中,仅过量未交联原花青素在一周内释放,而交联的PC在PC-dbpECM中保持稳定.1 mg/mLPC即可基本抑制体外钙化.PC或GA交联的dbpECM都有较好的血液相容性,但PC交联明显优于GA.2.5 mg/mL PC交联的dbpECM血小板的粘附率(7.8%)显著低于未交联组(26.1%)和GA交联组(36.6%),表明PC-dbpECM具有较好的抗血栓形成能力.结果表明PC-dbpECM具有良好的力学性能,稳定性和血液相容性,有可能成为制备人工心脏瓣膜的优良材料.In this study,decellularized bovine pericardium matrix（dbpECM） was cross-linked by using PC at series concentrations.Mechanical properties,stability and hemo-compatibility of PC-crosslinked dbpECM（PC-dbpECM） were investigated.The results show that the tensile strength of the PC-dbpECM which crosslinked by the 2.5 mg/mL PC is increased from 29.1 MPa to 51.3 MPa with the elastic modulus roughly at basic level.Resistance of PC-dbpECM to collagenase hydrolysis is equivalent to that of GA-crosslinked.When PC-dbpECM soaked in D-Hanks solution,only the excessive non-crosslinked PC released within a week,leaving the crosslinked PC stable all the experiment period.PC crosslinked dbpECM（1 mg/mL） can effectively inhibit calcification in vitro.Both PC- and GA-dbpECM have ideal hemo-compatibility, but the PC cross-linking is superior to that of GA.The rate of platelet adhesion on the surface of 2.5 mg/mL PC crosslinked dbpECM（7.8%） was significantly lower than that of noncrosslinked group（26.1%） and the GA crosslinked group（36.6%）,indicating that PC-dbpECM has low coagulation spotential.These data suggest that PC-dbpECM has good mechanical properties,stability and hemo-compatibility,suitable for preparing biological heart valves.

关 键 词：原花青素 脱细胞牛心包膜 力学性能 稳定性 血液相容性 

分 类 号：Q33[生物学—遗传学]