漂浮绿藻中缘管浒苔(Ulva linza)鉴定方法的探索(英文)  

An exploratory study on identification means of Ulva linza from floating green algae

作　　者：陈斌斌[1] 马家海[1] SHIN Jong-Ahm 高嵩[1] 

机构地区：[1]上海海洋大学农业部水产种质资源发掘利用重点实验室,上海201306 [2]全南大学校水产海洋大学,韩国丽水市556-901

出　　处：《华东师范大学学报（自然科学版）》2013年第5期71-79,共9页Journal of East China Normal University(Natural Science)

基　　金：国家海洋局公益项目(201205010);国家海洋局重点实验室开放研究基金(MATHAB00000000);科技部国家科技支撑计划(2012BAC07B03)

摘　　要：以研究漂浮绿藻中的缘管浒苔(Ulva linza)为出发点,根据绿藻的形态学特征,选取漂浮绿藻中具有U.linza重要特征的藻体进行形态学、分子生物学的分类学研究.结果显示,具有U.linza重要特征的漂浮藻体大多为LPP复合体(U.linza-procera-prolifera)类群(15个样品),而且采自江苏的DF05、山东的QD03以及浙江的XS01、XS02等4个固着类型的样品也在该分支中.Ulva flexuosa分支中有2个样品(均为采自福建的固着种类),Ulva compressa分枝包含了如东的4个漂浮样品.采自如东内河、射阳沿海池塘的漂浮藻体也均被包含在LPP复合体中.25个样品中只有4个被鉴定为U.linza,与其形态特征研究结果相符合.漂浮绿藻的形态学特征与分子生物学研究结果存在很大差别.这说明单纯地依靠传统分类学特征或分子生物学手段对漂浮绿藻中的缘管浒苔(U.linza)进行分类是不可取的,甚至对漂浮绿藻都不能适用.在同一地点采集到的漂浮绿藻中,大都存在不同形态特征的类型,经过分子生物学分析后被鉴定为不同的种类.根据ITS序列的系统进化树分析结果,绿潮藻中至少存在LPP复合体中的种类以及U.compressa.To study on morphological characteristics of Ulva linza from floating green algae, we selected specimens with the typical features of U.linza.Only 4 specimens from 25 samples were determined as U.linza from the morphological characteristics results.The molecular biology research by means of nuclear-encoded ITS sequences analysis was also carried out.Fifteen floating specimens with characters of U.linza clustered into the LPP（U. linza-procera-prolifera） complex group including the attached species DF05 from Jiangsu Province,QD03 from Shandong Province and XS01,XS02 from Zhejiang Province.The floating green algae from Rudong inland river and coastal pool of Sheyang were included in LPP complex as well.U.flexuosa group included two specimens that just were fixation growth algae from Fujian Province.U.compressa group had four specimens,and they all were floating green algae.A great disparity existed between the morphological characteristics and the molecular results.Identification of U.linza and other floating green algae based on either morphology or ITS sequence unilaterally was not reliable.Almost all of floating green algae from the same location had certain morphological types,and they were considered to be different species by the ITS sequences analysis.The result of N-J tree and ITS data showed that the species in LPP complex and U.compressa existed in the green tide.But U.flexuosa did not exist in the floating green algae in this research.

关 键 词：绿潮 缘管浒苔 形态学 ITS 分子生物学 

分 类 号：Q89[生物学]