孕晚期妇女B族链球菌PCR检测结果分析  

Analysis of group B streptococcus detection results by real-time polymerase chain reaction in 445 late pregnants

作　　者：黄永建[1] 陈波[1] 张勇[1] 刘剑荣 钟丽[1] 

机构地区：[1]江西省萍乡市人民医院,萍乡337055

出　　处：《江西医药》2013年第7期581-584,共4页Jiangxi Medical Journal

基　　金：江西省萍乡市科技局(2012)

摘　　要：目的探讨妊娠晚期孕妇B族链球菌带菌状态的检测方法。方法对在我院产检及分娩的孕妇445例妊娠晚期孕妇,取其阴道下1/3分泌物及肛周分泌物,应用细菌培养及实时PCR进行B族链球菌检测,并观察其妊娠结局。结果445例妊娠晚期孕妇B族链球菌细菌培养阳性17例,实时PCR阳性39例阳性率明显高于细菌培养。B族链球菌阳性孕妇的宫内感染、早产和产后出血发生率明显高于对照组(P<0.05)。结论实时PCR检测B族链球菌有较高的敏感度和特异性,是妊娠晚期孕妇常规检测B族链球菌感染的首选方法。Objective To explore the methods of detecting the Group B Streptococcus（GBS）. Methods Pick up the vaginal se- cretions and anal secretions in the 445 cases of late pregnant women of our hospital to test GBS through microbiological culture and real-time PCR, and observe the pregnancy outcome. Results The bacterial cuhure＇s positive cases of the 445 patients have 17 cases, which the real-time PCR＇s positive cases have 39 cases. The real-time PCR＇s positive rates were significantly higher than the bacterial culture＇s. The incidences of the intrauterine infection, premature delivery and postpartum haemorrhage in GBS positive pregnant women were significantly higher than the control group, and the differences have the statistical significance （P〈0. 05）. Conclusion The real-time PCR detection of GBS has a higher sensitivity and specificity than the microbiological culture, and it is the preferred method for GBS testing of the late pregnant women.

关 键 词：B族链球菌(GBS) 妊娠末期 妊娠结局 聚合酶链反应 微生物培养 

分 类 号：R711.73[医药卫生—妇产科学]