籼粳亚种间琼脂糖凝胶电泳多态性SSR标记筛选与评价  

Screening and Evaluation of Polymorphic SSR Markers by Agarose Gel Electrophoresis in Rice(Oriza sativa L.) Subspecies Indica and Japonica

作　　者：姚国新[1,2] 卢磊[1] 但志武[2] 黄文超[2] 

机构地区：[1]湖北工程学院生命科学技术学院,湖北孝感432000 [2]武汉大学生命科学学院,湖北武汉430072

出　　处：《河南农业科学》2013年第8期16-20,共5页Journal of Henan Agricultural Sciences

基　　金：湖北省教育厅重点项目(D20132704);湖北省自然科学基金项目(2012FFB00504);湖北工程学院博士科研启动基金项目(HE2010002)

摘　　要：利用琼脂糖凝胶为电泳介质,籼稻9311和粳稻日本晴为模板,从756对SSR标记中筛选能够分辨多态性的标记用于籼粳亚种遗传研究。共筛选到176对SSR标记,其在籼粳亚种中的多态性能够肉眼分辨,平均覆盖的遗传距离为10.2cM,并挑选部分SSR标记评价籼粳交群体遗传背景,带型分辨的效果良好。该套标记将使SSR标记的应用更加广泛快捷。In order to obtain the polymorphic markers for genetic research of rice subspecies,756 pairs of SSR markers were screened by agarose gel electrophoresis,using indica variety 9311 and japonica variety Nipponbare as the DNA template,of which 176 SSR markers showed the subspecific polymorphisms with an average genetic distance of 10.2 cM.Some markers were selected to evaluate the genetic background of indica and japonica rice,and the effect of band differentiation was good.The set of markers would allow the application of SSR markers more extensively and efficiently.

关 键 词：水稻 籼粳交 SSR标记 琼脂糖 

分 类 号：S511[农业科学—作物学]