不同培养基质对麦冬离体培养的影响  

Effects of Different Culture Medium on Ophiopogon japonicus (L.F) Kew-Gawl. in vitro Culture

作　　者：万学锋[1,2,3,4] 赖钟雄[3] 陈菁瑛[1,2] 

机构地区：[1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建省农业科学院药用植物研究中心,福建福州350003 [3]福建农林大学园艺植物生物工程研究所,福建福州350002 [4]福建省漳州市农业科学研究所,福建漳州363005

出　　处：《福建农业学报》2013年第7期670-674,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2011R1020-2)

摘　　要：为研究麦冬离体培养条件,以麦冬无菌试管苗为材料,比较不同铵态氮和硝态氮的比例及BA、NAA对麦冬试管苗增殖与生根的影响。结果表明,在有BA的条件下,硝态氮和铵态氮的比例以2.2∶1最合适,麦冬增殖系数为2.60;相同条件下,有BA培养基的麦冬增殖系数高于无BA培养基。不同氮素比例条件下麦冬生根情况也不同,在无NAA的条件下,硝态氮与铵态氮的比例为1.7∶1时,生根情况最好;在有NAA的条件下,生根情况与无NAA的变化趋势相似,但在硝态氮与铵态氮的比例为2.0∶1时,出现不生根的情况。The effects of NH＋ and NO;- levels and BA, NAA on vitro proliferation and rooting of Ophiopogon japonicus （L. F） Kew-Gawl were compared with vitro culture, Ophiopogon japonicus （L. F）Kew-Gawl to study the conditions of Ophiopogon japonicus （L. F） Kew-Gawl. The results showed that the coefficient of japonicus proliferation was 2.60 under the optimum ratio of NH＋ to NO was 2.2 ： 1 with BA. Further, the proliferation with BA was higher than that without BA at the same condition. In addition, the root proportions of different nitrogen conditions were also different. When the ratio of NH＋ to NO was 1.7 ： 1, the situation was the best for rooting without NAA. The trend of rooting was almost the same between with NAA and without NAA. But it was not rooting when the ratio of NH＋ to NO3- was 2 ： 1.

关 键 词：麦冬 离体培养 培养基质 

分 类 号：S682[农业科学—观赏园艺]