从烟草EST微卫星标记中筛选合子胚中优势表达的基因(英文)  

Polymorphic microsatellite markers from expressed sequences tag in Nicotiana tabacum for obtaining the genes of preponderant expression in zygotes

作　　者：张俊娥[1] 李芬[2] 孙蒙祥[3] 

机构地区：[1]江西师范大学生命科学学院,南昌330022 [2]河南师范大学生命科学学院,河南新乡453007 [3]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072

出　　处：《广西植物》2013年第4期456-459,555,共5页Guihaia

基　　金：江西省自然科学基金(2010GZN0109);江西省教育厅基金(GTT11386)

摘　　要：为获得烟草合子胚中的优势表达基因,利用CAP3程序对来自烟草公共数据库的EST序列进行组装,利用MISA程序从组装后的EST中筛选SSR位点,将多态性的SSR位点在烟草合子文库中进行扩增并对等位基因进行分析。结果表明:具有多态性的16个SSR标记中,有9个基因能从烟草的合子库中成功扩增得到。该研究为筛选烟草合子胚中优势表达基因提供新的途径。In order to obtain the genes of preponderant expression in zygote cells of tobacco,CAP3 program was used to assemble ESTs from GenBank,and MISA program were used to screen microsatellites. Then polymorphic microsatellites were amplified in cDNA of tobacco zygotes library. The results showed that sixteen polymorphic SSR markers from tobacco ESTs deposited in a public sequence database were developed. Of these,nine genes could be successfully amplified in cDNA of tobacco zygotes library. The study would provide new channel to screen genes of preponderant expression in tobacco zygotes.

关 键 词：EST SSR 优势表达基因 合子 烟草 

分 类 号：Q948[生物学—植物学]