人参果PVM病毒的克隆与鉴定  

Cloning and Identification of The PVM Virus of Ginseng Fruit

作　　者：张菲菲[1] 张金文[1] 高宜峰[1] 姚攀锋[1] 

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070

出　　处：《甘肃农业科技》2013年第8期18-20,共3页Gansu Agricultural Science and Technology

基　　金：甘肃省科技支撑计划"用融合基因dsRNA获得抗病性人参果种质资源的研究"(1011NKCA077)部分内容;甘肃省农牧厅农业生物技术专项"导入甜蛋白基因创造甜味人参果种质研究"(GNSW-2012-22)部分内容

摘　　要：通过对武威市凉州区人参果感病植株所表现出来的花叶、皱缩、植株矮小等症状观察,初步判断可能感染了马铃薯M病毒(PVM)。结合分子生物学手段,提取感病人参果叶片总RNA,采用RT-PCR方法扩增到一段长300 bp的基因序列,连接至PMD19-T载体并测序。测序结果同GeneBank中PVM CP基因序列[GI:361071296]同源性达到了98.97%,说明成功克隆到了PVM基因片段,即供试人参果确实感染了PVM病毒。The mosaic, wrinkled, small plant and other symptoms of susceptible plants of Liangzhou ginseng fruit were observed, the auditor should make a pre-judgment that may be infected with potato virus M （PVM）. Experiments combined with molecular biology methods and extract leaves total RNA of the patient feeling of ginseng fruit, it was amplified by RT-PCR method to a long 300 bp gene sequences and connected to PMD19-T vector and sequenced. The Sequencing results showed that homology reached 98.97%with the GeneBank PVM CP gene sequence [GI： 361071296], it can explain gene fragment was cloned into the PVM successful, that the test does ginseng fruit infected with PVM virus.

关 键 词：人参果 PVM病毒 克隆 鉴定 

分 类 号：S432.41[农业科学—植物病理学]