姜黄素对人微血管内皮细胞增殖、迁移、成管以及凋亡的影响  

Effect of Curcumin on Human Microvascular Endothelial Cell Proliferation, Migration, Tube Formation and Apoptosis in Vitro

作　　者：刘城[1] 吴平生[1] 王月刚[1] 裴静娴[1] 赖艳娴[1] 

机构地区：[1]广州市第一人民医院心血管内科,广东广州510006

出　　处：《广州中医药大学学报》2013年第5期681-684,773,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:30370587);广东省自然科学基金项目(编号:S2011040004458)

摘　　要：【目的】研究姜黄素对人微血管内皮细胞(HMVEC-L)增殖、迁移、成管以及凋亡的影响。【方法】将姜黄素分为0、20、40、80μmol/L 4个实验组,分别用非放射性细胞增殖检测(MTS)法、划痕实验、成管实验以及Hoechst 33258与TUNEL双重荧光法检测姜黄素对HMVEC-L增殖、迁移、成管以及凋亡的影响。【结果】MTS结果显示:从姜黄素与HMVEC-L共同培养的第1天开始,20～80μmol/L浓度组均可抑制HMVEC-L增殖,与对照组(0μmol/L)比较差异有统计学意义(P<0.05或P<0.01),尤以80μmol/L组抑制HMVEC-L增殖能力最显著(P<0.05或P<0.01)。划痕实验结果显示:20～80μmol/L姜黄素浓度组HMVEC-L迁移率均显著降低,各组HMVEC-L迁移率比较差异有统计学意义(P<0.05),其中尤以80μmol/L组HMVEC-L迁移率最低。成管实验结果显示:20～80μmol/L姜黄素浓度组HMVEC-L成管长度均显著降低,各组HMVEC-L成管长度比较差异有统计学意义(P<0.05),其中尤以80μmol/L组HMVEC-L成管长度最小。Hoechst 33258与TUNEL双重荧光染色结果显示:在姜黄素与HMVEC-L共同培养过程中HMVEC-L发生了凋亡。【结论】姜黄素可通过抑制HMVEC-L增殖、迁移、管样形成并诱导其发生凋亡,从而有效抑制肿瘤血管形成。Objective To study the effect of curcumin on the proliferation, migration, tube formation and apoptosis of human lung microvascular endothelial cells （HMVEC-L） in vitro. Methods The HMVEC-L were co-cultured with different concentrations of curcumin （0, 20, 40 and 80 μmol/L） , and then HMVEC-L proliferation, migration, tube formation and apoptosis were measured by non-radioactive MTS/PES assay, scratch adhesion test, Matrigel assay and Hoechst33258/TUNEL fluorescence staining, respectively. Results The results of non-radioactive MTS/PES assay showed that HMVEC-L proliferation was suppressed by 20 - 80μmol/L of curcumin, in particular by 80 μmol/L of eurcumin, from the first day of co-cuhure （P〈0.05 or P〈0.01 compared with the control group at 0 μmol/L） The results of scratch adhesion test showed that HMVEC-L migration rate was decreased by 20- 80 μmol/L of curcumin （P〈0.05）, and the rate was the lowest in curcumin group of 80 μmol/L. The co-culture of curcumin at 20 - 80 μmol/L also reduced the length of formative tube of HMVEC-L, and the effect of 80μmol/L of curcumin was the strongest （P〈0.05） Matrigel assay and Hoechst33258 /TUNEL fluorescence staining showed that migration and tube formation was suppressed by curcumin treatment, and the apoptosis of HMVEC-L was presented after the co-culture with curcumin. Conclusion Curcumin may play the role of tumor angiogenesis suppression through the inhibition of HMVEC-L proliferation, migration, tube formation and apoptosis.

关 键 词：姜黄素 药理学 人微血管内皮细胞 肿瘤 中药疗法 血管内皮细胞 病理学 细胞培养 

分 类 号：R282.5[医药卫生—中药学]