CDV、CPV和CPIV多重PCR检测方法的建立  

Establishment of a Multiplex PCR for Simultaneous Detection of CDV,CPV and CPIV

作　　者：康泰[1] 毕玉敏[1] 陈欣[1] 孙忠晟 王祖荣 尹燕博[1] 

机构地区：[1]青岛农业大学动物科技学院,山东青岛266109 [2]青岛澳兰百特生物工程有限公司,山东青岛266101 [3]青岛博隆实验动物有限公司,山东即墨266225

出　　处：《动物医学进展》2013年第9期62-65,共4页Progress In Veterinary Medicine

摘　　要：为了建立一种快速诊断犬瘟热病毒、犬细小病毒和犬副流感病毒的多重PCR方法,参照GenBank中的犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬副流感病毒(CPIV)基因序列,设计了3对引物分别用于特异扩增犬瘟热病毒F基因、犬细小病毒VP2基因和犬副流感病毒F基因上的目的片段。通过优化反应条件,建立同时扩增CDV(740bp)、CPV(419bp)和CPIV(559bp)的多重PCR方法。结果表明特异性和敏感性良好,对CDV、CPV、CPIV 3种病毒的最低核酸检测量为1ng/μL。临床样品的多重PCR检测结果表明,建立的多重PCR方法能够对CDV、CPV、CPIV单个感染或混合感染的临床样品进行快速鉴别诊断。.A novel multiple PCR assay was established for detection of canine distemper virus （CDV）, ca- nine parvovirus virus （CPV） and canine parainfluenza virus （CPIV）. According to CDV, CPV and CPIV gene sequences in GenBank,3 pairs of primers were designed and used to amplify CDV F, CPV VP2, CPIV F genes. Under the optimized conditions of multiplex PCR, three special fragments of CDV（740 bp） ,CPV （419 bp） and CPIV（559 bp） were obtained. The specificity and sensitivity results were satisfactory,and the minimum detection amouts of 3 virus were 1 ng/μL. The multiple PCR results of clinical samples showed that the multiplex PCR method is suitable for rapid diagnosis of clinical samples with CDV, CPV, CPIV single or mixed infections.

关 键 词：多重PCR 犬瘟热病毒 犬细小病毒 犬副流感病毒 

分 类 号：S854.43[农业科学—临床兽医学]