UPLC-MS/MS法测定猪尿中β_2-受体激动剂的基质效应研究  

Matrix Effects in the Residue Detection of β_2-agonists in Pig Urine by UPLC-MS/MS

作　　者：邱增枝[1] 郑增忍[2] 赵思俊[2] 曲志娜[2] 王君玮[2] 孙晓亮[2] 刘坤[2] 谭维泉[2] 邹明[1] 

机构地区：[1]青岛农业大学,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《动物医学进展》2013年第9期66-70,共5页Progress In Veterinary Medicine

摘　　要：为探讨猪尿中β2受体激动剂UPLC—MS／MS检测的基质效应及消除方法，将猪尿液样品经高氯酸酸解，乙酸乙酯和甲基叔丁基醚混合提取后，用MCX固相萃取柱净化，进行超高效液相色谱-串联质谱法检测。发现猪尿液中8种β2-受体激动剂UPLC-Ms／MS检测的基质效应为71．0％～114．5％，并比较基质添加标准曲线以及内标法两种定量方法，对基质效应进行消除研究。结果表明，5．0mL猪尿液在0．4、1．0、2．0ng／mL添加浓度下，内标法的定量结果为64．7％～136．0％。基质添加校正曲线的定量结果为77．9％-117．9％。基质添加校正曲线定量更加稳定，可以取代内标法进行定量。To investigate the matrix effects in the residue detection of β2-agonists in pig urine and elimina- ting methods, pig urine samples were acidolysised by perchloric acid, extracted with ethyl acetate and meth- yl tertiary butyl ether, the extracts were applied to MCX cartridge for cleanup, the separation was per- formed by ultra performance liquid chromatography-tandem mass spectrometry. 71.0%-114.5%levels of matrix effects were found in 8 β2-agonists detection. The internal standard method and matrix dilution cali- bration curve were compared to eliminate matrix effect. The results showed that,quantitative results of the internal standard method was 64. 7%-136.0%, matrix spiked calibration curve quantitative results was 77.9% -117.9%,with 5.0 mL pig urine at 0.4 ng/mL,1.0 ng/mL and 2.0 ng/mL standard added. The quantitative matrix spiked calibration curve was more stable, and internal standard method could be re- placed by matrix spiked calibration curve for quantitative.

关 键 词：Β2-受体激动剂 超高效液相色谱-串联质谱 基质效应 尿液 猪 

分 类 号：S859.797[农业科学—临床兽医学]