猫细小病毒的分离与鉴定  

Isolation and Identification of Feline Parvovirus

作　　者：刘碧涛[1] 任常宝 张晓战[1] 许冬蕾[1] 郑良焰[1] 唐兆新[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]肇庆大华农生物药品有限公司,广东肇庆526238

出　　处：《动物医学进展》2013年第9期124-127,共4页Progress In Veterinary Medicine

摘　　要：为丰富猫细小病毒(FPV)流行病学资料和制备灭活疫苗,通过猫肾传代细胞(F81)培养方法从疑似感染FPV病猫粪便中分离病毒。对分离到的病毒进行理化性质分析和FPV特异性检测引物PCR鉴定,然后对FPV的VP2基因进行扩增测序。理化性质分析结果与FPV特性均相符,VP2基因测序结果与GenBank已发表的FPV标准株VP2基因序列对比分析,核苷酸序列同源性达到99.0%,证明该分离毒株为猫细小病毒。该毒株的成功分离进一步充实了我国FPV病毒库,也为灭活疫苗的制备奠定了基础。In order to enrich the feline parvovirus epidemiological data and prepare the vaccine, a strain was success- fully isolated with the F81 cell culture method. After identification by the method of physicochemical properties and FPV specific primers, the nucleotides of VP2 were amplified by PCR and sequenced. The results of physicochemi- cal properties were all consistent with the characteristics of FPV, and the homologies of the VP2 gene sequences of the isolated strain and the FPV standard strain were 99.0%. The newly isolated FPV strain further enriched the FPV virus database, and laid the foundation for the preparation of inactivated vaccine.

关 键 词：猫细小病毒 分离鉴定 聚合酶链反应 

分 类 号：S852.659.2[农业科学—基础兽医学]