葡萄籽原花青素对大鼠脾脏淋巴细胞与肝细胞增殖的影响  

The Effects of Procyanidin from Grape Seed on the Splenic Lymphocytes and Hepatocytes Proliferation from Rat

作　　者：茅婷婷[1] 解玲娜[1] 刘畅[1] 闵婉平[1] 金凯[1] 王俊杰[1] 方洛云[1] 蒋林树[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国农学通报》2013年第23期10-14,共5页Chinese Agricultural Science Bulletin

基　　金：北京市自然科学基金项目"葡萄籽原花青素提高断奶仔猪免疫功能的机理研究"(6092006)

摘　　要：为了研究葡萄籽原花青素（Procyanidin from Grape Seed,GSP）对大鼠脾脏淋巴细胞与肝细胞增殖与活化的影响,揭示GSP提高机体免疫性能的作用机理,为后续的试验分组提供理论依据。试验采用不同终浓度的GSP培养液[0.00（对照）、0.05、0.10、0.50、1.00、5.00、10.00、15.00]与大鼠脾脏淋巴细胞,[0.00（对照）、0.05、0.10、0.50、1.00、5.00、10.00、15.00、20.00、25.00μg/mL]与大鼠肝细胞系IAR-20共育。在12、24、48h后,用酶标仪在490nm处测得各孔的OD值。结果显示：（1）添加0.05~5.00μg/mL的GSP促进淋巴细胞增殖,当浓度超过10.00μg/mL将抑制淋巴细胞增殖。（2）添加0.05~10.00μg/mL的GSP促进肝细胞增殖,当浓度超过20.00μg/mL将抑制肝细胞增殖。（3）一定范围内,GSP对淋巴细胞和肝细胞的促增殖作用有浓度和时间依赖性。葡萄籽原花青素能够促进体外培养大鼠的脾脏淋巴细胞和肝细胞增殖活性,具有提高细胞抗氧化和细胞免疫的作用。To study of the effects of GSP （Procyanidin from Grape Seed） on the Splenic Lymphocytes and hepatocytes proliferation from Rat, investigate the mechanism to improve immune performance of rats, provide a theoretical basis for the follow-up test group. The experiments used a single factor design. The splenic lymphocytes were treated with different concentration of GSP [0.00 （control group）, 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00 μg/mL ] and the hepatocytes were treated with different concentration of GSP [0.00 （control group）, 0.05, 0.10, 0.50, 1.00, 5.00, 10.00, 15.00, 20.00, 25.00 μg/mL ]. Procyanidins incubated with these cells to 12, 24 , 48 h, respectively. The plates were read at 490 nm. The results showed that： （1） for the lymphocytes of Splenic, the concentrates of GSP ranging from 0.05 μg/mL to 5.00 μg/mL had promoted effect to the lymphocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of splenic lymphocytes was inhibited; （2） for the hepatocytes IAR-20, the concentrates of GSP ranging from 0.05 μg/mL to 10.00 μg/mL had promoted effect to the hepatocytes proliferation, while the concentrates of GSP enhanced reach to 10.00 μg/mL, the proliferation of hepatocytes was inhibited; （3） Within a certain range, GSP exhibited concentration and time dependent stimulative effects on the proliferativeactivity of splenic lymphocytes and hepatocytes. GSP could promote the spleen lymphocytes and liver cells proliferation which cultured in vitro from Rat. So GSP had promoted effect to cellular antioxidant ability and immune function of the cells.

关 键 词：葡萄籽原花青素 淋巴细胞 肝细胞系 增殖 大鼠 

分 类 号：S852.1[农业科学—基础兽医学]