除草剂阿特拉津在刺参中的生物富集与消除效应研究  

Research on accumulation and elimination of Atrazine in Apostichopus japonicus

作　　者：徐英江[1,2] 宫向红[1,2] 任传博 田秀慧[1,2] 刘慧慧[1,2] 刘京熙[1,2] 孙岩[1,2] 王茂剑[1,2] 

机构地区：[1]山东省海洋水产研究所,烟台264006 [2]山东省海洋生态修复重点实验室,烟台264006 [3]山东省烟台山水海产有限公司,烟台264006

出　　处：《海洋渔业》2013年第3期337-342,共6页Marine Fisheries

基　　金：山东省现代农业产业技术体系刺参产业创新团队资助;烟台市科技发展计划项目资助(2012134号);山东省科学技术发展计划项目资助(2012GHY11517号);水生动物营养与饲料泰山学者岗位(2007-2012)资助

摘　　要：采用超高效液相色谱-串联质谱法进行了除草剂阿特拉津在刺参(Apostichopus japonicus)中生物富集及消除规律的研究。采用半静态水质接触暴露法,在15±1℃水温条件下,将刺参分别浸浴于阿特拉津浓度1.0、10.0和200μg.L-1水中进行富集效应实验。结果显示,在3个暴露浓度下,阿特拉津在刺参中的富集速度较快,随暴露质量浓度的增加,富集量逐渐增加,呈正相关关系;分别在第1天、第2天和第4天达到富集最大值,富集最大值分别为1.77、14.6和246μg.kg-1;最大富集系数分别为1.77、1.46和1.23。阿特拉津在刺参中的残留量变化趋势均表现为先增加后降低,并最终维持在某一质量浓度水平(上述暴露质量浓度下分别为0.67、1.58和168μg.kg-1)而仅产生微小波动。实验结果表明,富集系数随暴露质量浓度的增加而减小,呈负相关关系,说明在低质量浓度条件下,刺参更容易对阿特拉津产生富集作用。将达到稳定状态的刺参转移至洁净水中进行消除实验,低质量浓度下,分别在第2天和第10天降至未检出,在高暴露质量浓度下,从第7小时至第46天共取样14次,其浓度范围为2.81±0.64μg.kg-1。本研究所得的药时曲线可为刺参阿特拉津污染的净化提供数据支持。Accumulation of Atrazine in Apostichopus japonicus under laboratory conditions was studied using ultra performance liquid chromatogram tandem mass spectrometry. The Apostichopus japonicus was divided randomly, then exposed to water containing 1.0 μg L-1, 10.0 μg L-1 and 200 μg L-1 Atrazine at 15 1 C. After exposure, Apostichopus japonicus was placed into clean water for the subsequent elimination experiment. The accumulation amount in three concentrations increased with the increase of Atrazine concentration, presenting significantly positive correlation. The highest accumulation value （Cmax ） for the three concentrations were 1.77 μg kg-1 ,14.6 μg kg-1 and 246 μg kg-1 when placed for 1 d, 2 d and 4 d, and the biggest bioconcentration factors （BCF） were 1.77, 1.46 and 1.23. The residues of Atrazine first increased and then decreased, and finally remained at certain concentrations with slim variations. After the accumulation experiments, the residues were 0.67 p.g ~ kg-I ,1.58 p^g ~ kg-1 and 168 txg ~ kg-I for the three concentrations, respectively. DAS 2. 0 pharmacokinetic parameter calculation program was used to process Atrazine content-time data and to calculate the relevant pharmacokinetic parameters. Results showed that the BCF decreased with the increase of the Atrazine concentration, presenting significantly inverse correlation, indicating that Atrazine could be easily accumulated at lower concentration. When Apostichopus japonicus was bred in clean water, the Atrazine accumulated was eliminated rapidly. Atrazine was not detected on 2 d and 10 d at 1.0 μg L-1 and 10.0 μg L-1 At 200 μg L-1 , it was determined for 14 times from 7 h to 46 d, showing 2.81 0.64 μg kg-1 of Atrazine in Apostichopus japonicus. The accumulation and elimination curves of Atrazine in Apostichopus japonicus provided useful information for studying decontamination of aquatic organisms contaminated by Atrazine contamination.

关 键 词：阿特拉津 刺参 富集 消除 超高效液相色谱-串联质谱法 

分 类 号：S948[农业科学—水产养殖]