女贞子提取物对大强度耐力训练大鼠不同组织Mg^(2+)-ATPase活性的影响  

Effect of Fructus Ligustri Lucidi Extracts on Mg^(2+)-ATPase Activity in Different Tissues of Rats of High-Intensity Endurance Training and Exercise Capacity

作　　者：马云慧[1] 熊正英[2] 

机构地区：[1]宝鸡文理学院体育系,陕西宝鸡721013 [2]陕西师范大学运动生物学研究所,陕西西安710062

出　　处：《内蒙古师范大学学报（自然科学汉文版）》2013年第4期494-498,共5页Journal of Inner Mongolia Normal University(Natural Science Edition)

基　　金：陕西省自然科学基金资助项目(2010JM2014);陕西省体育局体育科研项目(11005)

摘　　要：分析大鼠服用女贞子提取物(Fructus Ligustri Lucidi extracts,FLLE)进行大强度耐力训练后,心、肝、脑、肾和股四头肌组织中Mg2+-ATPase活性的变化,研究FLLE作为运动补剂对大鼠运动能力的影响.把大鼠随机分为安静对照组、运动对照组和运动+FLLE组,每组8只.运动对照组进行6周大强度跑台训练,运动+FLLE组除大强度跑台训练外,每天灌服2mL 400mg/kg的FLLE,安静对照组和运动对照组每天灌服2mL 0.5%Tween-80溶液.6周后,安静组在安静时取材,运动组和运动+FLLE组进行一次力竭性运动后取材,分别测定不同组织的Mg2+-ATPase活性.实验结果显示:运动组和运动+FLLE组大鼠不同组织的Mg2+-ATPase活性均显著低于安静组,运动+FLLE组大鼠不同组织的Mg2+-ATPase活性均高于运动组(P<0.01或P<0.05);运动组大鼠与安静组相比较,其心、肝、脑、肾和股四头肌组织中Mg2+-ATPase活性分别下降21.01%、10.06%、11.15%、19.89%和12.36%;运动+FLLE组大鼠与运动组相比较,其心、肝、脑、肾和股四头肌组织中Mg2+-ATPase活性分别升高21.62%、9.21%、8.24%、21.94%和7.05%;运动+FLLE组大鼠力竭运动时间比运动对照组延长23.09%.这说明补充FLLE可以提高机体内抗氧化剂的浓度,防止细胞膜的氧化损伤,维持细胞内离子浓度稳态,保证线粒体内物质代谢和能量代谢的正常进行,使能量维持在一个较高的水平.To analyze the change of Mg2+-ATPase activity in different tissue of rats like heart,liver,brain,kidney and quadriceps after the rats were fed with Fructus Ligustri Lucidi extracts(FLLE) and trained with high-intensity endurance training.To study the effect of FLLE as the exercise nutrition tonifying formula on the exercise capacity in rats.SD rats were randomly divided Sedentary control group,exercise control group and exercise+FLLE group,n=8.Exercise control group received high-intensity treadmill training for 6 weeks,exercise+FLLE group was fed with 2 mL 400 mg/kg FLLE extract besides high-intensity treadmill training daily.Sedentary control group and exercise control group was given with 2 mL 0.5% Tween-80 solution.After 6 weeks,sedentary control group was in rest state,and after exercise control and exercise+FLLE group were given an exhaustive exercise,determination of the different tissue Mg2+-ATPase activity of each group.The results show the exercise control group and exercise+FLLE group of the different tissue Mg2+-ATPase activity was significantly lower than the sedentary control group,exercise+FLLE group of the different tissue Mg2+-ATPase activity was higher than the exercise control group(P<0.01 or P<0.05);with the sedentary control group,exercise control rat heart,liver,brain,kidney and quadriceps Mg2+-ATPase activity decreased by 21.01%,10.06%,11.15%,19.89% and 12.36%;with the exercise control group,exercise+FLLE group rat heart,liver,brain,kidney and quadriceps Mg2+-ATPase activity were increased by 21.62%,9.21%,8.24%,21.94% and 7.05%;compared with that of rats in the control group,the time of exhaustive exercise of rats in the exercise+FLLE group was prolonged by 23.09%.That indicates,complementing FLLE can increase the concentration of antioxidants in the body,preventing oxidative damage to cell membranes,maintaining the steady-state concentrations of intracellular ion,and ensuring the mitochondria metabolism and energy metabolism in normal,so that energy can be maintained at a high level.

关 键 词：女贞子提取物 Mg2+-ATPase活性 组织 运动能力 大鼠 

分 类 号：G804.22[文化科学—运动人体科学]