贵港最大面积原生地新收集普通野生稻分子鉴定评价  

Molecular Identification of New Collecting Resources of O. rufipogon Griff from the Largest Original Habitat in Guigang, Guangxi Province

作　　者：黄娟[1] 陈成斌[1] 杨庆文[2] 徐志健[1] 梁云涛[1] 张万霞[2] 梁世春[1] 

机构地区：[1]广西农业科学院水稻研究所,南宁530007 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国农学通报》2013年第24期11-16,共6页Chinese Agricultural Science Bulletin

基　　金：广西农科院基本科研专项"广西新收集野生稻资源遗传多样性分子鉴定评价"(桂农科2011YZ04);国家重点保护野生植物普查及重要作物野生近缘植物调查与收集项目"广西野生稻调查与收集"(2130135-1101);农作物种质资源保护项目"野生稻保存复壮;编目与利用"(NB2012-2130135-15);科技部国家农作物种质资源平台;国家野生稻种质资源平台(南宁)运行服务;广西自然科学基金专项"野生稻抗白叶枯病新抗源筛选与渗入系的培育"(2010GXNSFA013086);广西科学研究与技术开发计划项目(桂科攻1123001-3A)

摘　　要：为了对来自贵港市最大面积原生地新收集普通野生稻资源进行遗传多样性分析,并在此基础上进一步对居群内的个体进行区分。选择分布于水稻染色体组的25对微卫星(SSR)引物对来自贵港的349份材料进行多样分析和遗传结构研究。结果表明,所有个体平均等位基因数A=6,有效等位基因数Ae=2.23,期望杂合度He=0.44,实际杂合度Ho=0.40,香农指数I=0.88。该居群可分为4个亚群结构,并且90%以上个体可以得到区分。该群体遗传多样性丰富,SSR分子标记可以简单有效的区分群体内的大部分个体,提高了保护效率。To estimate the genetic diversity of common wild rice from the Largest Original Habitat in Guigang, Guangxi and further to distinguish the individuals within the population. Twenty-five SSR loci distributed on the twelve chromosomes of rice were used to study the genetic diversity and structure of 349 wild rice individuals. The results showed that, the mean number of allele （A） was 6 and the effective number of allele （Ae） was 2.23. Expected heterozygosity （He） was 0.44, and observed heterozygosity （Ho） was 0.40 as well as the Shannon index （I） was 0.88. The population could be divided into four subsets structure, and more than 90% of individuals could be distinguished. It had abundant genetic diversity within the population and SSR molecular markers was the effective method to distinguish efficiency of wild rice. most of individuals in the group also to improve protection

关 键 词：普通野生稻 遗传多样性 分子标记 

分 类 号：S3[农业科学—农艺学]