草茎点霉毒素分离及除草活性研究  

Separation and Herbicidal Activity Bioassay of Toxins from Phoma herbarbum

作　　者：纪明山[1] 李思嘉[1] 谷祖敏[1] 靳强[1] 吴玉星[1] 张丽[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866

出　　处：《中国农学通报》2013年第24期193-196,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"草茎点霉毒素Ⅲ对鸭跖草致病机理研究"(31071746)

摘　　要：为开发防治鸭跖草的新型生物除草剂,从草茎点霉中分离纯化除草毒素并对其组分的活性进行了初步研究。利用乙酸乙酯浸提获得草茎点霉粗毒素,通过TLC、柱层析和HPLC法对茎点霉粗毒素进行分离,并采用针刺法测定分离组分的除草活性。结果表明,经TLC和柱层析分离后,得到8组组分,其中6号组分活性最高,病斑面积达到10.48mm2。6号组分经HPLC分析后,得到5组馏分,其中4号馏分活性最强,病斑面积达到12.69mm2。In order to develop new bioherbieides for Commelina eommunis control, crude toxin of Phoma herbarum was separated and purified, and its herbicidal activities was analyzed. After extracted by ethyl acetate, the crude toxin was separated with TLC, silica column chromatography and HPLC. The herbicidal activity was tested by pinpricking inoculation at seedling stage. Among the eight components obtained by silica column chromatography and TLC separation, the herbicidal activity of No. 6 component was the highest with 10.48 mm2 lesion area. Five fractions were separated from the No. 6 component by HPLC, No. 4 fraction showed the highest activity. The lesion area reached to 12.69 mm2.

关 键 词：草茎点霉 毒素 分离 除草活性 

分 类 号：S482.7[农业科学—农药学]