端粒酶RNA模板定点突变对肿瘤细胞Bcap-37的作用研究  

Research on Effect of Telomerase RNA Template Site-specific Mutagenesis on Cancer Cell Bcap-37

作　　者：韦敬航[1] 杨力媛[1] 郑洁[1] 吕齐[1] 何丹枫[1] 刘小川[1] 

机构地区：[1]浙江理工大学生物工程研究所,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2013年第5期753-757,共5页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省大学生科技创新活动计划(新苗人才计划)

摘　　要：端粒酶在正常体细胞中极少表达,而在大多数人类恶性肿瘤和细胞系中高度表达,这暗示着端粒酶活性可能与肿瘤密切相关。本研究以人乳腺癌细胞株Bcap-37接种裸鼠形成的肿瘤组织块为实验材料,通过检测端粒酶活性、端粒长度及细胞凋亡的情况,观察端粒酶RNA模板定点突变的作用。结果显示:MT-hTER组样本的端粒酶活性低于对照组,端粒长度短于对照组,细胞核形态不够稳定,有较多细胞凋亡的现象,而对照组细胞核形态稳定,较少发生细胞凋亡。以上结果提示着端粒酶RNA模板定点突变在一定程度上改变了人乳腺癌细胞株Bcap-37的端粒酶活性、端粒长度及细胞凋亡发生率。Telomerase is rarely expressed malignant tumor and cell lines. This implies This research observes the effect of telomerase RNA template site-specific mutagenesis by detecting telomerase activity, telomere length and cell apoptosis with tumor tissue block formed in nude mouse inoculated with human mammary cancer cell line Bcap-37 as the experimental material. The result shows that samples in MT-hTER group have a lower telomerase activity and shorter telomere length than the control group; the karyomorphism is not stable enough and there is cell apoptosis; however, in the control group, the karyomorphism is stable and cell apoptosis rarely occurs. Results above indicate that telomerase RNA template site-specific mutagenesis changes the telomerase activity, telomere length and cell apoptosis occurrence rate of human mammary cancer cell line Bcap-37 to a certain extent.

关 键 词：人乳腺癌细胞株Bcap-37 端粒酶活性 端粒长度 细胞凋亡 

分 类 号：Q243[生物学—细胞生物学]