毛花猕猴桃氯仿萃取物抗肿瘤活性研究  

Study on Antineoplastic Activity of Chloroform Extraction of Actinidia Eriantha Benth

作　　者：郭辉辉[1] 应帅[1] 王晓明[1] 金秀芳[1] 马丛丛[1] 许传莲[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2013年第5期758-761,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

摘　　要：对毛花猕猴桃根甲醇提取物进行初步分离,对所得氯仿萃取物活性部位进行定性以及体外抗肿瘤活性研究。使用系统溶剂法分离毛花猕猴桃根甲醇提取物,获得氯仿萃取物。使用显色反应对氯仿萃取物进行初步定性。以紫杉醇为阳性对照药,通过MTT法检测毛花猕猴桃根甲醇提取物的氯仿萃取活性部位对肝癌细胞株BEL-7404增殖的抑制作用。结果表明毛花猕猴桃氯仿萃取物的主要成分可能为生物碱类物质。氯仿萃取物对BEL-7404有明显的增殖抑制作用并呈剂量依赖性关系,其IC50为114.8μg/mL。This study preliminarily separates methanol extract of actinidia eriantha benth root, determines the nature of active site of chloroform extraction obtained and studies invitro antineoplastic activity. It separates methanol extract of actinidia eriantha benth root with systematic solvent method and obtains chloroform extraction, preliminarily determines the nature of chloroform extraction by using chromogenic reaction and detects the inhibiting effect of active site of chloroform extraction of methanol extract of actinidia eriantha benth root on liver cancer cell line BEL-7404 proliferation with MTT method with TAX as positive control medicine. The result shows that the main component of chloroform extraction of actinidia eriantha benth might be alkaloid substance. Chloroform extraction has significant proliferation inhibiting effect on BEL-7404 and presents dosage dependency. Its IC50 is 114. 8μg/mL.

关 键 词：毛花猕猴桃 系统溶剂法 氯仿萃取物 MTT法 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]