电焊工血液重金属含量与外周血DNA损伤的相关性  

Correlation between blood concentrations of heavy metals and DNA damage in peripheral blood from welders

作　　者：姚春冀[1] 谭玉凤[1] 金凌之[1] 刘克澄[1] 宋鹏[1] 宋杨[1] 高明[1] 吴南翔[1] 楼建林[1] 

机构地区：[1]浙江省医学科学院卫生学研究所,杭州310013

出　　处：《国际流行病学传染病学杂志》2013年第4期222-226,共5页International Journal of Epidemiology and Infectious Disease

基　　金：国家自然科学基金（81001242）;浙江省自然科学基金（Y2100687）;浙江省医药卫生骨干人才计划（2011RCA001）;浙江省医学支撑学科建设计划（11-ZC02）

摘　　要：目的研究电焊工血液中重金属含量及其与DNA损伤之间的关系。方法抽取22名来自医疗器械制造企业（A组）、25名来自船舶制造企业（B组）的电焊工以及22名管理人员（对照组）的外周静脉血，用电感耦合等离子体质谱仪（ICP-MS）检测血液中铬（Cr）、锌（Zn）、镉（Cd）、铅（Pb）、钻（Co）、镍（Ni）、锰（Mn）的含量，并用彗星试验检测外周血DNA损伤。用多元线性回归法及相关性分析法分析重金属含量与DNA损伤之间的关系。结果与对照组相比，A，B组电焊工血液中Pb和Mn含量均明显增高，差异有统计学意义（A组：Upb=136．0，tMn=-6．2；B组：Ueb=78．5，tMn=．7．6，P〈0．01），A组电焊工血液中Cr、Ni含量较高，差异有统计学意义（Ucr=64．0，tNi=-4．4，P〈0．01）；B组电焊工血液中Cd含量较高，差异有统计学意义（U=260．0，P〈0．01）。多元线性回归分析显示，控制B组与对照组饮酒史的差异因素后，B组电焊工外周血DNA损伤仍显著高于对照（β=3．9，SE=0．9，t=4．3，P〈0．01）。但相关性分析显示，两组电焊工血液中各重金属含量与外周血DNA损伤均无显著相关性（P〉0．05）。结论电焊工血液中的重金属负荷种类可能与电焊丝的材料有关，但本研究并未发现单一重金属负荷与外周血DNA损伤存在相关性。B企业外周血DNA损伤可能与其他未明因素有关。Objective To investigate the concentrations of heavy metals in peripheral blood from welders and its relationship with blood DNA damage. Methods The blood samples were collected from 22 welders of the medical equipment manufacture enterprise （group A）, 25 welders of shipbuilding enterprise （group B） and 22 administrators （control group）. The concentrations of chromium （Cr）, zinc （Zn）,cadmium （Cd）, lead （Pb）, cobalt （Co）, nickel （Ni）, and manganese （Mn） in blood were detected by inductively coupled plasma-mass spectrometry（ICP-MS）. The DNA damage of peripheral blood was measured by comet assay. The relationship between metal concentrations and the levels of DNA damage was analyzed by multiple linear regression and correlation analysis. Results Compared with control group, blood concentrations of Pb and Mn were higher in group A and B （group A： Upb = 136.0, tMn = -6.2; group B： UPb = 78.5, tMn = -7.6; P all 〈 0.01）, blood levels of Cr and Ni were significantly higher in group A（ Ucr = 64. 0, tNi = -4.4; P 〈 0.01）and blood concentrations of Cd were higher in group B respectively（ Ucd = 260.0, P 〈 0.01）. The results of multiple linear regression analysis indicated that the level of DNA damage in B group were significantly higher than the control group after the control of drinking factor （β = 3.9, SE = 0.9, t = 4.3, P 〈 0.01）. However the results of correlation analysis indicated that there was no correlation between blood concentrations of heavy metals and the levels of DNA damage （ P 〉 0.05）. Conclusions Differences of heavy metal burdens in blood from welders may be related to the steel wires used in welding. But no correlation between DNA damage and any single metal burden in blood is found in this study. The blood DNA damage observed in group B may attribute to other unclear factors.

关 键 词：金属 重 DNA损伤 电焊工 

分 类 号：R131[医药卫生—劳动卫生]