再生障碍性贫血外周血Th17和CD4^+ CD25^+ Treg细胞表达情况研究  

Expression of Blood Th17 and CD4^+ CD25^+ Treg Cells in Patients with Aplastic Anemia

作　　者：童来根[1] 吴文忠[1] 周志刚[1] 张云平[1] 陈亚峰[1] 黄文娟[1] 许欢[1] 苏倩倩[1] 

机构地区：[1]宜兴市人民医院血液内科,江苏宜兴214200

出　　处：《中国实验血液学杂志》2013年第4期974-978,共5页Journal of Experimental Hematology

摘　　要：本研究旨在探讨成人再生障碍性贫血(aplastic anemia,AA)外周血Th17和CD4+CD25+调节性T细胞(Treg)表达情况。45例初发AA患者分为轻型AA(n=25)和重型AA(n=25)两组,15例正常人作为对照。应用流式细胞术检测AA患者外周血中Th17和CD4+CD25+Treg的比例,ELISA检测血清及刺激后外周血单个核细胞(PBMNC)上清液中的白介素(IL-17)、γ干扰素(IFN-γ)及肿瘤坏死因子-α(TNF-α)水平,并与正常对照组比较。结果表明:重型AA(SAA)组外周血Th17细胞比例,血清中IL-17及IFN-γ表达高于轻型AA(MAA)和正常人组,而重型AA组外周血CD4+CD25+Foxp3+Treg细胞比例降低。与轻型AA和正常对照相比,重型AA患者单个核细胞培养后上清液中IL-17及IFN-γ表达水平显著增加。AA患者血红蛋白计数与Th17细胞及血清IL-17表达呈负相关,与CD4+CD25+Treg表达成正相关。结论:重型AA患者外周血Th17细胞应答增强,而CD4+CD25+Treg细胞缺乏,贫血的严重程度与外周血Th17/Treg免疫应答失衡密切相关。This study was aimed to investigate the expression of blood Th17 and CD4＋ CD25＋ regulatory T cells（Treg） in the patients with aplastic anemia（AA）.Forty-five patients with AA were enrolled into this study,and were divided into mild aplastic anemia（MAA） group（n=25） and severe aplastic anemia group（SAA）（n=20）,blood cell count was recorded.15 healthy donors were enrolled as control.Proportions of blood Th17 and CD4＋ CD25＋ Treg cells were determined by flow cytometry.The serum levels of IL-17,IFN-γ and TNF-α,as well as their concentrations in culture supernatant of phytohemagglutinin（PHA）-stimulated peripheral blood mononuelear cells,were measured by ELISA.The results showed that the proportions of blood Th17 cells and concentration of blood serum IL-17 and IFN-γ increased in patients with SAA,compared with MAA and normal controls,but CD4^＋CD25^＋Foxp3^＋ Treg cells obviously decreased in patients with SAA.The concentrations of IL-17 and IFN-γ significantly increased in culture supernatant of SAA group.Hemoglobin level in the patients with AA negatively correlated with the population of Th17 cells and serum IL-17 level,whereas positively correlated with the expression of CD4^＋CD25^＋Treg cells.It is concluded that the increased response of Th17 cells and deficiency of CD4^＋CD25^＋ Treg cells present in severe aplastic anemia.The severity of anemia may be related with the imbalance between Th17 and CD4^＋CD25^＋Treg cell response.

关 键 词：再生障碍性贫血 Th17细胞 CD4+CD25+调节性T细胞 IL-17 IFN-γ TNF-α 

分 类 号：R331.143[医药卫生—人体生理学] R457.1[医药卫生—基础医学]