HLA-E基因的多态性对同胞全相合造血干细胞移植后巨细胞病毒感染的影响  

Influence of HLA-E Polymorphism on Cytomegalovirus Infection after HLA-matched Hematopoietic Stem Cell Transplantation

作　　者：朱子玲[1] 吴小津[1] 吴德沛[1] 冯宇锋[1] 徐杨[1] 马骁[1] 韩悦[1] 刘跃均[1] 李彩霞[1] 

机构地区：[1]苏州大学附属第一医院血液科,江苏省血液研究所,卫生部血栓与止血重点实验室,江苏苏州215002

出　　处：《中国实验血液学杂志》2013年第4期990-994,共5页Journal of Experimental Hematology

基　　金：国家自然科学青年科学基金项目(编号81100390);江苏省自然科学基金面上项目(编号BK2011265);江苏省临床医学中心(编号ZX201102);江苏省医学创新团队与领军人才项目资助(编号LJ201138)

摘　　要：本研究旨在探讨人类非典型HLA抗原E(HLA-E)对同胞全相合造血干细胞移植(HSCT)后巨细胞病毒(CMV)感染的影响。以2005年1月至2011年1月在我院行HLA同胞全相合HSCT患者119例为研究对象,采用PCR和测序方法检测供、受体HLA-E基因多态性。结果表明,HLA-E*0101/0101纯合型占20.17%,HLA-E*0103/0103纯合型占27.73%,HLA-E*0101/0103杂合型占52.10%;HLA-E*0101/0101纯合子组CMV感染15例,CMV感染率为62.50%;HLA-E*0103/0103纯合子组CMV感染16例,CMV感染率为48.48%;HLA-E*0101/0103杂合子组CMV感染20例,CMV感染率为32.25%。含HLA-E*0103基因组与HLA-E*0101/0101纯合子组患者CMV感染率比较,差异有统计学意义(P=0.0295),与CMV疾病的发生率比较,差异仍有统计学意义(P=0.0074)。结论:HLA-E基因多态性对同胞全相合移植后CMV感染和疾病的发生存在影响。This study was aimed to explore the potential association of HLA-E polymorphism with the incidence of cytomegalovirus（CMV） infection after HLA-matched hematopoietic stem cell transplantation,119 HLA-genoidentical sibling pairs for HLA-E polymorphism were analyzed,HLA-E DNA was amplified by polymerase chain reaction（PCR）,and the amplified DNA products was also sequenced directly after purification to confirm the genotype.The results showed that the homozygous HLA-E＊0101/0101 accounted for 20.17%,the homozygous HLA-E＊0103/0103 accounted for 27.73%;heterozygous HLA-E＊0101/0103 accounted for 52.10%;in homozygous HLA-E＊0101/0101 group,15 cases were infected with CMV and the CMV infection rate was 62.50%;in homozygous HLA-E＊0103/0103 group,16 cases were infected with CMV and the CMV infection rate was 48.48%;in heterozygous HLA-E＊0101/0103 group 20 cases were infected with CMV and the CMV infection rate was 32.25%.As compared with the homozygous HLA-E＊0101/0101 group,the CMV infection rate in HLA-E＊0103 group displays statistical significance（P=0.0295）.The CMV infection rate occured higher and its significance is statistical（P=0.0074）.It is concluded that the HLA-E gene polymorphism is associated with CMV infection after HLA-genoidentical bone marrow transplantation.

关 键 词：造血干细胞移植 巨细胞病毒 HLA-E基因分型 

分 类 号：R457.7[医药卫生—治疗学]