IL-1β促进脐带间充质干细胞的造血支持作用  

IL-1β Promotes the Hematopoietic Support of Human Umbilical Cord Mesenchymal Stem Cells

作　　者：及月茹[1] 杨舟鑫[1] 李丽娜[2] 韩之波[1,3] 池颖[1] 韩忠朝[1,3,2] 

机构地区：[1]中国医学科学院北京协和医学院血液学研究所实验血液学国家重点实验室,天津300020 [2]暨南大学医学院,广东广州510632 [3]中国医学科学院血液学研究所泰达生命科学技术研究中心,天津300457

出　　处：《中国实验血液学杂志》2013年第4期1005-1009,共5页Journal of Experimental Hematology

基　　金：国家重大科学计划“973计划”(编号2011CB964800);“863计划项目”(编号2011AA020118)

摘　　要：本研究旨在探讨IL-1β对人脐带间充质干细胞(hUC-MSC)的造血支持的影响。将分离得到的hUC-MSC以2×106接种于75 cm2培养瓶中,贴壁2 h后,加入10 ng/ml IL-1β培养36 h后收集培养上清和细胞。观察有/无IL-1β的条件培养液对CD34+细胞造血支持的影响;real time PCR检测加/不加IL-1βMSC的造血相关因子mRAN的变化;ELISA法检测造血相关因子的差异。结果表明,含有IL-1β的MSC条件培养液能明显增强CD34+细胞集落形成能力,且以粒系和粒-单核集落形成单位为主。IL-1β促进了MSC GM-CSF、G-CSF、IL-6的mRNA的表达,促进GM-CSF、G-CSF、IL-6蛋白自脐带MSC的分泌。结论:IL-1β能够增强MSC的造血支持能力,尤其是向髓系分化的能力。This study was aimed to investigate the effect of IL-1β on hematopoietic support of human umbilical cord mesenchymal stem cells（hUC-MSC）.2×10^6 hUC-MSC were seeded in 75 cm2 flasks,after adherence to wall for 2 h,10 ng/ml IL-1β was added in hUC-MSC supernatant and cultured for 36 h,then the culture supernatants and cells were harvested.The effect of conditioned medium with/without IL-1β on CD34^＋ cell hematopoietic support was observed,mRNA expression changes of hUC-MSC cultured in medium with/without IL-1β were monitored by real time PCR,the differences in hematopoiesis-related factors were detected by ELISA.The results showed that the conditioned culture medium of hUC-MSC with IL-1β enhanced the ability to form colony of CD34^＋ cells,especially CFU-G and CFU-GM in vitro;IL-1β promoted the mRNA expression of GM-CSF,G-CSF,IL-6 on MSC;IL-1β also promoted the secretion of GM-CSF,G-CSF,and IL-6 protein from hUC-MSC.It is concluded that IL-1β enhances hematopoietic support capacity especially,capability of MSC to myeloid differentiation.

关 键 词：IL-1Β 人脐带间充质干细胞 CFU 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]