白介素12对辐射损伤小鼠的防护作用  

Radioprotective Effect of rmIL-12 on Mice Irradiated by γ-Ray

作　　者：王利[1,2] 翟瑞仁 逄朝霞 张超 余长林[1] 

机构地区：[1]军事医学科学院附属医院血液科,北京100071 [2]解放军401医院崂山分院血液肿瘤科,山东青岛266101

出　　处：《中国实验血液学杂志》2013年第4期1027-1031,共5页Journal of Experimental Hematology

基　　金：全军科技攻关课题编号NO08g157

摘　　要：本研究旨在探讨重组鼠白介素12(rmIL-12)对辐射损伤小鼠的防护作用。56只雄性BALB/c小鼠均给予60Coγ线6.0 Gy一次全身照射,然后随机分为照射对照组rmIL-12治疗组和重组鼠血小板生成素(rmTPO)治疗组。rmIL-12治疗组小鼠于照射前24 h腹腔注射rmIL-12(分为5、20μg/kg 2个剂量组),rmTPO治疗组于照射后30 min和24 h分别皮下注射rmTPO 15μg/kg,对照组给予等体积的无菌PBS。每日2次观察小鼠一般情况,每3d检测1次外周血细胞数,分别于照射后14和28 d收集骨髓细胞进行集落培养。结果表明,rmIL-12治疗组小鼠一般情况较对照组改善,外周血血小板(Plt)下降速度慢于对照组,Plt恢复时间较对照组明显提前(11 d∶14 d),且Plt最低值明显高于对照组(15.9%∶8.1%,18.2%∶8.1%,P<0.01);rmIL-12治疗组白细胞(WBC)恢复速度快于对照组;rmIL-12 5μg/kg组与rmTPO组WBC、Plt恢复速度相当,20μg/kg组的WBC、Plt恢复速度快于5μg/kg组及rmTPO组(P>0.05)。骨髓有核细胞集落培养显示,照射后14和28 d rmIL-12治疗组CFU-Mix明显高于对照组(P<0.01),与rmTPO治疗组比较差异无统计学差异(P>0.05),28 d rmIL-12 5μg/kg组和rmTPO组CFU-GM均高于对照组(P<0.05),但低于20μg/kg组(P<0.05),两组间差异无统计学意义(P>0.05)。结论:rmIL-12对辐射损伤小鼠具有明显的防护作用。The aim of this study was to investigate the radioprotective effect of recombinant murine interleukin 12（rmIL-12） on mice irradiated by γ-ray.Fifty-six BALB/c mice were totally irradiated by 6.0 Gy of 60Co γ-ray and randomly divided into irradiation control group,rmIL-12 treated group and recombinant murine thrombopoietin（rmTPO） treated group.The 5 and 20 μg/kg of rmIL-12 were administrated intraperitoneally at 24 h before irradiation respectively（low and high dose rmIL-12 treated group）,15 μg/kg of rmTPO was administrated subcutaneously at 30 min and 24 h following irradiation in rmTPO treated group.The general conditions of mice were observed twice a day,the changes in body weight and peripheral blood cell counts were examined once every three days,bone marrow cells were collected to perform colony cultivation at day 14 and 28 after irradiation.The results showed that the general conditions of mice in rmIL-12 treated group were better than those in irradiation control group.Compared with the irradiation control group,5 and 20 μg/kg rmIL-12 treatment significantly promoted platelet recovery,resulting in less profound nadirs（15.9% vs 8.1%,18.2% vs 8.1%,P〈0.01） and rapid recovery to normal levels（11 days vs 14 days）.WBC count recovery rate in rmIL-12 treated group was faster than that in the irradiation control group.The WBC and platelet count recovery rate in 5 μg/kg rmIL-12 treated group were as fast as that in the rmTPO treated group,both of which were slower than that in 20 μg/kg rmIL-12 treated group（P〈0.05）.Semi-solid bone marrow cell culture also demonstrated that rmIL-12 could stimulate bone marrow cells to form more CFU-Mix than those in the irradiation control group in vitro at day 14 and 28 after irradiation（P〈0.01）.There was no significant difference between rmIL-12 and rmTPO treated groups（P〈0.05）,CFU-GM counts in 5 μg/kg rmIL-12 treated group and rmTPO treated group at day 28 after irradiation were higher than those in irradiation control group（P〈0.

关 键 词：白细胞介素12 血小板生成素 辐射损伤 造血损伤 辐射防护 Γ射线 

分 类 号：R818.05[医药卫生—放射医学]