阿司匹林对人脐血晚期内皮祖细胞功能的影响  

Effect of Aspirin on Function of Human Umbilical Cord Blood-Derived Late Endothelial Progenitor Cells

作　　者：刘珍珍[1,2] 李国强[3] 刘萌[4] 孙晟轩[3] 安广宇[5] 董宁征[1,2] 

机构地区：[1]苏州大学附属第一医院,江苏省血液研究所,江苏苏州215006 [2]卫生部血栓与止血重点实验室,江苏苏州215006 [3]苏州大学附属第二医院骨外科,江苏苏州215004 [4]苏州大学唐仲英血液学研究中心,江苏苏州215123 [5]首都医科大学附属北京朝阳医院肿瘤科,北京100020

出　　处：《中国实验血液学杂志》2013年第4期1032-1037,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(面上项目)(项目批准号:81070415)

摘　　要：本研究旨在观察阿司匹林对人脐血晚期内皮祖细胞(endothelial progenitor cells,EPC)功能的影响。采用密度梯度离心法及免疫磁珠分选法从脐血获得CD34+的单个核细胞,将其接种在人纤维连接蛋白包被的培养板上,培养2周后收集贴壁细胞,通过流式细胞术、免疫荧光、RT-PCR、DiI-Ac-LDL的摄取及体外血管形成能力等方法对其进行鉴定。用不同浓度阿司匹林(终浓度分别为0.1,1,10,100,1 000,10 000μmol/L)处理EPC 24 h,然后分别采用CCK-8比色法、Transwell小室、黏附能力测定实验来观察EPC的增殖能力、迁移能力和黏附能力的变化。结果表明:低浓度的阿司匹林(0.1-1 000μmol/L)对脐血晚期EPC的增殖无明显影响,但可增强晚期EPC的黏附及迁移能力,而高浓度的阿司匹林(10 000μmol/L)显著抑制晚期EPC的增殖和迁移,但对其黏附能力无明显影响。结论:低浓度的阿司匹林可增强晚期EPC的黏附及迁移能力,而高浓度的阿司匹林抑制晚期EPC的增殖、黏附及迁移能力,从而抑制内皮细胞的生成。This study was aimed to investigate whether aspirin has effect on function of late endothelial progenitor cells（EPC）.Cord blood CD34＋ cells were purified using the ficoll density gradient centrifugation and human CD34 positive selection kit,then the cells were inoculated on fibronectin-coated culture plate.After culture for 2 weeks,adherent cells were identified as EPC by flow cytometry,immunofluorescence,RT-PCR,uptake of Dil-Ac-LDL and matrigel tube formation assay.EPC were treated with different concentrations of aspirin（0.1,1,10,100,1 000,10 000 μmol/L） for 24 h,then the proliferation,adhesion and migration ability of these cells were analyzed by CCK-8 assay and transwell methods.The results indicated that the low concentrations of aspirin（0.1 and 1 000 μmol/L） promoted late EPC adhesive and migratory capacity,but no obvious effect on proliferation of late EPC were observed.On the other hand,the high concentrations of aspirin（10 000 μmol/L） inhibited proliferation and migratory capacity of EPC,but had no obvious effect on adhesive ability of EPC.It is concluded that low concentration of aspirin promotes migration and adhesion of late EPC,while the high concentration of aspirin decreases EPC proliferation and migratory capacity of EPC.

关 键 词：阿司匹林 内皮祖细胞 脐血 

分 类 号：R453[医药卫生—治疗学]