Identification and Characterization of Cotton Genes Involved in Fuzz-Fiber Development  

作　　者：Gaskin Wang Guo-Hong Zhao Yin-Hua Jia Xiong-Ming Du 

机构地区：[1]State Key Laboratory of Cotton Biology,Institute of Cotton Research of the Chinese Academy of Agricultural Sciences

出　　处：《Journal of Integrative Plant Biology》2013年第7期619-630,共12页植物学报（英文版）

基　　金：supported by grants from the National Key Basic Research and Development Program of China(2010CB12600)

摘　　要：Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in Gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.Cotton fuzz fibers, like Arabidopsistrichomes, are elongated unicells. It is postulated that a transcriptional complex of GLABRA1 （GL1）, GLABRA3 （GL3）, and TRANSPARENT TESTAGLABRA1 （TTG1） might be in existence in Arabidopsis as evidenced by their physical interaction in yeast, and the complex regulates expression of GLABRA2 （GL2） controlling trichome cell differentiation; it is also assumed that TRIPTYCHON （TRY） and CAPRICE （CPC） counteract the complex formation in neighboring cells. Here, the homologs GaMYB23 （a homolog of GL1）, GaDEL65 （a homolog of GL3）, GaTTG1, GaCPC and GaTRY were identified in Gossypium arboreum. We show that GaMYB23 can bind to and activate the promoters of GaCPC, GaGL2 and GaTRY, and that GaMYB23, GaTRY and GaTTG1 could interact with GaDEL65 in yeast and in planta. In situ analysis showed that GaMYB23, GaGL2, GaDEL65, and GaTRY were predominantly expressed in fuzz fiber, but GaTRY proteins were primarily found in undeveloped epidermal cells. A G. arboreum fuzzless mutant with consistently high level GaMYB23 transcript has lost the detectable GaMYB23-promoter of GaGL2 complex, corresponding to sharply reduced transcription of GaGL2. Our results support that cotton homologs to the genetic molecules regulating Arabidopsis trichome differentiation interacted in the epidermis of ovules and the redundant GaMYB23 serves as a negative regulator in fuzz-fiber patterning.

关 键 词：Convergent evolution fuzz  fuzzless mutant Gossypium arboreum lateral inhibitor trichome. 

分 类 号：TS117[轻工技术与工程—纺织材料与纺织品设计] S562[轻工技术与工程—纺织科学与工程]