HPLC法测定结香中山奈酚的含量  

Determination of Kaempferol in Edgeworthia chrysantha by HPLC

作　　者：刘元[1,2] 宋志钊[1,2] 

机构地区：[1]广西中医药研究院,广西南宁530022 [2]广西中药质量标准研究重点实验室,广西南宁530022

出　　处：《中国民族民间医药》2013年第15期14-14,16,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：广西科技攻关项目(桂科攻11107010-3-4)

摘　　要：目的:建立结香中山奈酚的含量测定方法。方法:采用高效液相色谱法,以XB-C18色谱柱(4.6mm×250mm,5μm)为分离柱,以甲醇-0.5%磷酸(57:43)为流动相,366nm为检测波长,柱温为室温。结果:山奈酚在9.9～158.4μg/mL范围内,峰面积与其浓度呈良好线性关系(r=0.9999),平均回收率为103.0%,RSD为0.78%(n=6)。结论:该方法简便、准确、重复性好,可作为该药材含量测定方法。Objective： To assay the content of Kaempferol in Edgeworthia chrysantha by HPLC. Method： The XB - C18 （4. 6 mm× 250mm, 5μm） column was used, the mobile phase consisted of Methanol： -0. 5% phosphoric acid （57 ： 43）, the detection wave length was 366nm and the column temperature was room temperature. Results： Linearity of Quercitr was good at 9. 9 -158.4μg/ mL （r =0. 9999）. The average recovery of buddleodide was 103.0% with RSD less than 0. 78% （n =6）. Conclution： The method is simple, accurate and repeatable, which can be applied in determination of Kaempferol in Edgeworthia chrysantha.

关 键 词：结香 山奈酚 HPLC 

分 类 号：R284.2[医药卫生—中药学]