阿特拉津降解酶固定化条件优化  

Optimization for the immobilized conditions of atrazine degradation enzyme

作　　者：张颖[1] 葛世杰[1] 姜昭[1] 梁海晶[1] 王溪[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2013年第8期23-28,共6页Journal of Northeast Agricultural University

基　　金：国家"十二五"农村领域国家科技计划(2011BAD04B02-1);黑龙江省科技攻关项目(GC12C20);哈尔滨市科技创新人才研究专项基金(2012RFXXN013)

摘　　要：采用海藻酸钠、聚阴离子纤维素作为载体对阿特拉津降解酶进行固定,通过单因素试验并应用主成分分析法确定海藻酸钠浓度、聚阴离子纤维素的浓度、pH和温度等因素对固定化酶的降解效果影响较大。采用正交试验确定固定化酶的最佳条件为:海藻酸钠浓度1.75%,聚阴离子纤维素浓度1.4%,温度29℃,pH 7.5。Degradation enzyme of atrazine was immobilized on the sodium alginate, PAC supporter. The single factor tests of immobilized condition were made through investigating atrazine degradation rate. Then the software SPSS determine the sodium alginate concentration, PAC concentration, pH and temperature were more important impact factors of the immobilized degradation enzyme effect. Orthogonal experiment commence between these factors which were selected by SPSS. The optimal condition of the immobilized： the concentration of sodium alginate was 1.75% and PAC was 1.4%. The optimal temperature for the immobilized atrazine degradation enzyme was 29℃; the optimal pH was 7.5.

关 键 词：固定化 阿特拉津降解酶 海藻酸钠 聚阴离子纤维素 正交试验 

分 类 号：F323.7[经济管理—产业经济]