离子束介导玉米DNA导入水稻引起遗传变异的RAPD分析  

RAPD Analysis of Hereditary and Variation of Maize Rice Developed by Ion Beam Mediated Transferring Maize DNA into Rice

作　　者：李红[1] 吴丽芳[1] 宋道军[1] 余增亮[1] 吴跃进[2] 

机构地区：[1]中国科学院等离子体物理研究所,230031 [2]安徽省农科院水稻研究所,230031

出　　处：《激光生物学报》1999年第4期261-265,共5页Acta Laser Biology Sinica

基　　金：重大自然科学基金!(19890300);国家重点科技攻关项目资助!(96-538-02-01)

摘　　要：本实验采用４０ 个随机引物对低能离子束介导紫玉米全ＤＮＡ 转化水稻早籼２１３ 获得的８ 个玉米稻株系基因组ＤＮＡ 进行ＲＡＰＤ检测。其中３６ 个随机引物得到扩增图谱。统计分析图谱中的各类扩增带，其中变异株系与早籼２１３ 的相似率为９１．２—９５．５％ 。差异带占总带数的８．９—１７．７％ ，说明外源ＤＮＡ 导入受体细胞引起后代基因组ＤＮＡ 的显著变异，这些变异很可能是表型及生理性状变异得以稳定遗传的物质基础。Eight maize rice line, developed by low energy ion beammediated transferring maize DNA into rice EI213, have stronger root system and exhibit purple red character of maize in shoot, glumal tip and stigma. Also the photosynthetic efficiency of maize rice have increased by averaged 84% compared with that of the EI213. Genomes of the 8 maize rice, original EI213 and maize were analyzed by RAPD with 40 10mer primers. Polymorphic fingerprints were obtained from 36 primers. Different kinds of amplified bands in RAPD patterns were calculated and analyzed, the similarity of maize rice and EI213 ranged 91.295.5%. The variant bands between maize rice and EI213 counted for 8.917.7% of the total bands of each line. The result indicated that exogenous DNA introduced into rice induced remarkable variation in progeny genomes. Those hereditary changes in plant phenotype and physiology may due to the genomes variation.

关 键 词：玉米稻 基因组DNA 遗传 RAPD检测 离子束介导 

分 类 号：S511.035[农业科学—作物学] Q78[生物学—分子生物学]