五种品系猪亲缘关系的RAPD分析  

Analysis on Phylogenetic Relationship and Selection of Polymorphic Primers to Five Breeds of Pigs by RAPD of 190 Arbitrary Primers

作　　者：吴开平[1] 吴丰春[1] 魏泓[1] 王爱德[2] 甘世祥[3] 曾养志[4] 

机构地区：[1]第三军医大学实验动物中心,重庆400038 [2]广西大学动物科学技术学院,南宁530005 [3]贵阳中医学院实验动物研究所,贵阳550002 [4]云南农业大学动物遗传资源研究室,昆明650201

出　　处：《遗传》2000年第4期217-220,共4页Hereditas（Beijing)

基　　金：国家"九五"重点科技攻关项目!(96A -23-06-01);国家重点基础研究发展规划项目!(G2000016106);国家自然科学基金!重点项目(398301

摘　　要：应用190个RAPD引物对贵州小型猪,巴马小型猪、西双版纳近交系小耳猪JB和JS亚系、荣昌猪Ⅰ系、长白猪的亲缘关系进行了初步分析,其中39个引物的扩增结果具有非常明显的品系间多态性,将其扩增结果以RAPDistancepackageVersion1 04程序进行分析,计算相对遗传距离指数1 -F,再用NJ法进行聚类分析,构建系统树。结果表明,三种小型猪相互间亲缘关系较近,尤以贵州小型猪与巴马小型猪更近。荣昌猪Ⅰ系和长白猪关系较近,但与小型猪关系较远。The phylogenetic relationship of five species of pigs including Guizhou miniature pig,Bama pig,Xisuangbanna inbred pig,Rongchang Pig and Landrance was studied by RAPD analysis.Thirty nine single polymorphic primers were selected out of 190 primers.The amplified fragments of thirty nine primers were analysed by the RAPDistance package version 1.04 and the phylogenetic tree was constructed using NJ method.The results indicated as the following: three strains of miniature pigs have close phylogenetic relationship and the phylogenetic relationship between Guizhou miniature pig and Bama miniature pig was much closer.Landrance and Rongchang pig have close phylogenetic relationship too,but their phylogenetic relationship is far from the three strains of miniature pigs.

关 键 词：随机扩增多态DNA 小型猪 亲缘关系 RAPD 

分 类 号：Q953[生物学—动物学]