（动物模型）肝移植免疫耐受的研究以及三维重建技术在肝泡状棘球蚴治疗中的应用：泡状棘球蚴感染对大鼠肝移植物存活的影响及机制探讨  

The study of the influence of hepatic alveolar echinococcus on the survival rates of OLT model

作　　者：李涛[1] 卡米力·吾拉木 王晓嵘 李瑾[4] 白磊[1] 温浩[1] 赵晋明[1] LI Tao1, Kamili Wulamu2 , WANG Xiaorong2 , LI Jin, BAI Lei, WEN Hao , ZHAO Jinming （ 1 Department of Vascular Surgery Center, Organ Transplantation Research Institute, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China ; 2 Hetian City People＇s Hospital, Hetian 848000, China; 3No. 69006 Medical Corp , Urumqi 830001, China; 4 Department of Anesthesia, The Frist Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China）

机构地区：[1]新疆医科大学第一附属医院消化血管外科中心肝脏腹腔镜外科,乌鲁木齐830054 [2]新疆和田市人民医院,新疆和田848000 [3]69006部队卫生队,乌鲁木齐830001 [4]新疆医科大学第一附属医院麻醉科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2013年第9期1225-1229,1241,共6页Journal of Xinjiang Medical University

基　　金：基金项目：国家自然科学基金（30760239）;新疆维吾尔自治区包虫病基础医学重点实验室开放课题（xJDX0202-2011-06）

摘　　要：目的探讨泡状棘球蚴感染对大鼠肝移植物存活的影响及其可能的机制。方法建立BN大鼠泡状棘球蚴感染模型，将实验大鼠分为实验组（LEW大鼠→感染泡球蚴3个月的BN大鼠）和对照组（健康LEW大鼠→健康BN大鼠），利川蚁袖套发建立肝移植模型，分别在移植术后第1、3、5、7天取肝脏组织，采用免疫组织化学的方法检测肝移植后排斥反应；采用流式细胞仪检测外周血中T淋巴细胞标志物CD4＋、CD8、CD28＋的表达，利用半定量RT-PCR的方法检测趋化凶子Fractalkine（FKN）的表达。结果实验组移植物存活时间[（15．5±3．93）d]明显长于对照组[（4．75±1．58）d]，差异有统计学意义（P〈0．05）；免疫组化结果示实验组排斥反应较对照组出现较晚且程度较轻；实验组CD4＋、CD8＋及CD28-的表达[（41．35±4．58）％、（21．94士2．61）％、（17．76土4．16）％]较对照组[（51．28±6．36）％、（33．85±3．02）％、（23．08±4．74）％]低，差异有统计学意义（P〈0．05）；实验组FKNmRNA表达较埘照组降低，差异有统计学意义（P〈0．05）。结论泡状棘球蚴感染能够延长大鼠肝移植物的存活，泡状棘球蚴感染对宿主免疫系统尤其对效应T淋巴细胞进行抑制是可能的途径之一。Objective To study the technique and complication of orthotopic liver transplantation with E. m infected rats and observe the immune rejection of liver transplantation. Methods 24 Lew rats which were the recipient were randomly assigned to two groups. Group A was transplanted with the infected Lew re- cipient （the Lew were infected E. m 3 monthes later）. Group B was transplanted group with the uninfected Lew recipient. ＂Two-cuff method＂ is used to build the model. The liver tissue is taken on the 1st , 3nl, 5th, and 7th day after the transplantation. Immunohistochemistry method was used to detect liver transplanla tion rejection; using flow cytometry to test the expression of T lymphocyte markers CD4＋ , CD84 , CD28＋ in peripheral blood; the use of semi quantitative RT-PCR method to detect chemokine Fractalkine （FKN） expression. Results The survival time of group A was （15.50±3.93） days while the control group （4.75±1. 58） days, and the difference was statistically significant （P 〈0.05）; immunohistochemical results show rejection of the experimental group is later and milder than in the control group; in the experimental group expression ofCD4＋ , CD8＋ and CD28＋ ＋（41. 35±4. 58） ＋ , （21.94±2.61）＋, （17.76±4.16）＋＋ is lower than the control group [（51.28±6.36）%, （33.85±3.02）%, （23.08±4.74）%], and the difference is statistically significant （P 〈0.05） ; FKN mRNA expression gets lower in the experimental group than the control group, the difference is statistically significant. Conclusion Survival time of Lew with AE was prominently longer than normal Lew. We will get new way to study liver transplantation with infected re cipient and to study the tolerance of liver transplantation. The model can be a new choice for the immuno logical study of live transplantation.

关 键 词：泡状棘球蚴 大鼠原位肝移植 免疫耐受 

分 类 号：R33[医药卫生—人体生理学;医药卫生—基础医学]