鲨鱼软骨血管生成抑制因子的纯化和功能  

Purification and Functional Characterization of a Shark Cartilage Factor Inhibitory to Angiogenesis

作　　者：沈先荣[1] 吉冬梅[2] 贾福星[1] 邓新贤[2] 孙加红[1] 胡忻然[2] 任大明[2] 

机构地区：[1]海军医学研究所,上海200433 [2]复旦大学遗传学国家重点实验室

出　　处：《生物化学与生物物理学报》2000年第1期43-48,共6页

基　　金：总后九五科研基金;上海市科学技术发展基金

摘　　要：以中国东海鲸鲨软骨为原料， 通过盐抽提、丙酮分级沉淀、离子交换层析、分子筛层析、高效液相色谱等步骤， 获得鲨鱼软骨血管生成抑制因子Ｉ（ｓｈａｒｋ ｃａｒｔｉｌａｇｅａｎｇｉｏｇｅｎｅｓｉｓｉｎｈｉｂｉｔｏｒｙ ｆａｃｔｏｒＩ，ＳＣＡＩＦＩ）， 对其分子量、抑制血管生成及抑制肿瘤生长活性进行了研究。结果显示ＳＣＡＩＦＩ分子量为１８ ｋＤ， 在细胞和整体水平上显著抑制新血管生成， 显著抑制小鼠肿瘤的生长。SCAIF I, an inhibitor of angiogenesis from shark cartilage was purified to homogeneity. The 4 mol/L guanidinium chloride extract of shark cartilage was fractionally precipitated with 35%—65% acetone, then purified by Resource Q ion exchange chromatography, Sephacryl S 300 gel filtration, and reverse phase high performance liquid chromatography. The pure inhibitor was homogeneous as a single band on a silver stained 15% sodium dodecyl sulfate polyacrylamide gel. SCAIF I had an molecular weight of 18 kD. It specifically inhibited proliferation of endothelial cells, and strongly blocked endothelial cell movement and angiogenesis in the chorioallantoic membrane of chick embryos. Systemic administration of SCAIF I at the dose of 5 mg/kg·d suppressed 87.93% of the growth of Lewis lung carcinoma implanted in C57BL/6 mice.

关 键 词：鲨鱼 软骨 血管生成抑制因子 肿瘤防治 SCAIF-I 

分 类 号：R965[医药卫生—药理学]