平胸龟Sox基因的克隆和序列分析  

The Clone and Sequence Analysis of Sox Gene in the P.megacephalum

作　　者：聂刘旺[1] 单祥年[2] 郭超文[1] 汪鸣[1] 张海军[1] 鲁晓萱 

机构地区：[1]安徽师范大学生物系,中国安徽芜湖241000 [2]南京师范大学生物系,中国江苏南京210097

出　　处：《激光生物学报》2000年第2期106-109,共4页Acta Laser Biology Sinica

基　　金：安徽省和江苏省自然科学基金资助课题!( 974 1 2 0 0 2 and 9750 61 1 2)

摘　　要：本文采用 PCR技术扩增和克隆了平胸龟的 Sox基因 ,并通过银染测序进行了序列分析。结果显示 ,平胸龟雌雄个体均能扩增出相同长度的片段 ,其 DNA序列与人 SRY基因的 HMG box同源性达 72 .7% ,推测的氨基酸序列与 SRY基因的 HMG box序列同源性为 4 6.5% ,充分显示出 Sox基因在系统进化上的保守性。本文为 Sox基因的起源和进化研究提供了资料。The Sox gene of P. megacephalum were amplified and clond using PCR. The amplification fragment was sequenced by PCR silversequencing. The results indicated that the Sox gene fragment can be amplified in P. megacephalum of male and female. The homologous of Sox gene sequences comparing with HMGbox of SRY is 72.7% , and the sequence homologous of diving amino acid is 46.5%. This may be concluded that Sox gene is conservative in phylogenetic. This paper provide the date for Sox gene study of origin and evolution.

关 键 词：SOX基因 序列分析 PCR 平胸龟 

分 类 号：Q959.630.3[生物学—动物学]