薄层培养的应用现状与前景  

The Application and Prospect of Thin Cell Layer Culture

作　　者：王鸿鹤[1] 黄学林[1] 

机构地区：[1]中山大学生命科学学院,广州510275

出　　处：《植物学通报》1999年第6期631-635,共5页Chinese Bulletin of Botany

摘　　要：植物薄层培养 (TCL)实验系统是 70年代初发展起来的一个新颖精致的实验系统。由于薄层培养具有组织结构简单、对调控因子敏感、培养易成功等优点 ,目前已经在植物细胞的分裂、分化及植物生长、发育的调控机制与分子机理等方面的研究得到了广泛的应用。本文综述了薄层培养在形态发生、细胞学、分子生物学等研究领域的应用现状与进展 ,并对当前薄层培养存在的问题作了评述。The plant Thin Cell Layer (TCL) culture system is a novel system developed in early 1970's. Sensitive to the regulaing factors and easy to use, TCL has been used on the studies of cell division and differentition, mechanism of plant growth and development. The latest progresses and application of TCL in morphogenesis, cytology and molecular biology are reviewed. A brief description is given on problems to be solved in the use of TCL.

关 键 词：薄层培养 形态发生 细胞学 分子生物学 植物 

分 类 号：Q943.1[生物学—植物学]