2,4-D诱导的花生体细胞胚发生的组织学研究  

A Histological Study on Somatic Embryogenesis of Embryonic Leaflets of Peanut (Arachis hypogaea L.) Induced by 2,4-D

作　　者：肖显华[1] 林荣双[1] 王庆华[1] 王顺珍[1] 

机构地区：[1]烟台大学生化系,烟台264005

出　　处：《植物学通报》1999年第6期691-695,共5页Chinese Bulletin of Botany

基　　金：山东省自然科学基金

摘　　要：花生成熟胚胚叶在MS附加 2 0mg/L 2 ,4_D的培养基上诱导 2 0d后 ,转移至无激素培养基MS0 继续培养 ,可获高频体细胞胚发生。组织学观察表明 ,体细胞胚起源于胚叶上表皮及表皮下数层细胞 ,这些细胞脱分化形成细胞质浓厚、细胞核大的胚性细胞团 ,胚性细胞团继续分裂形成体细胞胚。体细胞胚的发育过程经历球形胚、心形胚、鱼雷胚。High frequency somatic embryogenesis was obtained from embryonic leaflets of peanut (Arachis hypogaea L.) which were induced on MS medium containing 20 mg/L 2,4_D for 20 d and later subcultured on MS medium without phytohormones. Histological observations reavealed that somatic embryos originated from the cells of epidermis and several layers of subepidermis which underwent dedifferentiation and developed into embryonic cell mass with dense cytoplasm and large nucleus. The embryonic cell mass continued to devide and developed further into embryos through globular, heart_shaped, torpedo_shaped and cotyledon stages.

关 键 词：花生 体细胞胚发生 组织学研究 

分 类 号：S565.201[农业科学—作物学] Q943.1[生物学—植物学]