普通小麦与东方旱麦草异附加系和异代换系的选育与原位杂交检测  

Isolation and Verification of Triticum aestivum-Eremopyrum orientale Addition Lines and Substitution Lines by GISH

作　　者：张桂芳[1] 刘建文 黄远樟 丁敏 唐顺学[2] 贾旭[2] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国科学院遗传研究所,北京100101

出　　处：《Acta Genetica Sinica》2000年第1期50-55,共6页

基　　金：国家自然科学基金!39670459

摘　　要：旱麦草属（Ｅｒｅｍｏｐｙｒｕｍ）是用于小麦品种改良的又－潜在的植物资源。为了筛选小麦－旱麦草异附加系、异代换系，对普通小麦品种 Ｆｕｋｏｈｏ×东方旱麦草属间杂种的 ＢＣ２Ｆ３代材料的９６粒种子进行了染色体数目的检测，共检出１５粒２ｎ＝４３的种子， ８粒 ２ｎ＝ ４４的种子，进一步对以上材料进行的基因组ＤＮＡ原位杂交，共鉴定出３个单体附加系，２个二体附加系，１个双单体附加，１个小麦三体单体附加，１个附加３条东方旱麦草染色体的小麦单体，在染色体数为４２的个体中，检测出１个单体代换，１个双单体代换。根据ＢＣ２Ｆ３代自交品系来源的不同，初步认为由双单体附加自交比单体附加自交选择异附加系的效率高。Eremopyrum is a potential genus in wheat improvement To breed T aestivum-Er. orientale additional and substitutional lines, chromosome number of 96 BC2F3 individuals were accounted. fifteen plants with 2n=43 and eight plants with 2n=44 were checked out. GISH results of the individuals with 43 chromosomes showed that they blonged to three different monosomic addihons. Two disomic addihons, one double-monosomic addition, one trible-monosomic addition and one monosomic addition were found in the 2n=44 individuals. One double--monosomic substitution and one monosomic subshtution were obtained. We also proved that it is relatively easier to obtain addition lines in the selfing population than to obtain double-monosomic additions.

关 键 词：旱麦草 普通小麦 附加系 代换系 GISH 

分 类 号：Q343[生物学—遗传学]