猪肾细胞系IBRS-2感染猪细小病毒的检测  

Detection of porcine parvovirus infection in porcine kidney cell line IBRS-2

作　　者：李斌[1,2] 梁家幸[1,2] 苏乾莲[1,2] 赵武[1,2] 秦毅斌[1,2] 何颖[1,2] 卢冰霞[1,2,3] 段群棚[1,2] 李莹莹[1,2,3] 姜佳佳[1,2,3] 梁保忠[1,2] 

机构地区：[1]广西兽医研究所,广西南宁530001 [2]广西畜禽疫苗新技术重点实验室,广西南宁530001 [3]广西大学动物科学技术学院,广西南宁530005

出　　处：《中国动物检疫》2013年第8期67-69,74,共4页China Animal Health Inspection

基　　金：广西畜禽疫苗新技术重点实验室系统性研究课题(12-071-28-A-5);广西自然科学基金项目(2011GXNSFB018032);广西基本科研业务费专项(桂科专项13-1);公益性行业(农业)科研专项(201103008)

摘　　要：本研究应用聚合酶链式反应技术对未接种猪细小病毒,但出现类似猪细小病毒感染病变的日常培养猪肾细胞系IBRS-2进行检测,结果发现IBRS-2细胞感染了猪细小病毒。检测结果表明猪肾细胞系IBRS-2感染猪细小病毒较为常见,提示在IBRS-2细胞培养过程中要注意预防猪细小病毒的感染。In the study, porcine parvovirus （PPV） was detected in daily cultured porcine kidney cell line IBRS-2, which had not been inoculated with PPV, but showed PPV cytopathic, by polymerase chain reaction （PCR） . The detection result showed that these IBRS-2 cells were infected by PPV. Detection result suggested that porcine kidney cell line IBRS-2 infected by PPV was common. We should pay attention to prevention of PPV contamination in cell culture.

关 键 词：聚合酶链式反应 猪细小病毒 猪肾细胞系IBRS-2 检测 

分 类 号：Q813.11[生物学—生物工程] S852.659.2[农业科学—基础兽医学]