日本血吸虫卵黄培养细胞超微结构动态的研究  

ULTRASTRUCTURE OF CULTURED VITELLINE CELLS FROM ADULT SCHISTOSOMA JAPONICA

作　　者：董惠芬[1] 蒋明森[1] 杨孟祥[1] 杨明义[1] 李瑛[1] 周述龙[1] 

机构地区：[1]湖北医科大学寄生虫学教研室,武汉430071

出　　处：《水生生物学报》2000年第1期25-29,共5页Acta Hydrobiologica Sinica

基　　金：湖北省基金;省教委资助;武汉市青年科技晨光计划

摘　　要：从超微结构水平研究日本血吸虫卵黄细胞培养的动态。在体外培养过程中，卵黄细胞对外界环境的改变比日本血吸虫的其它体细胞更加敏感。随着培养时间的延长，卵黄细胞发生不同程度的变性，成熟卵黄细胞比未成熟卵黄细胞先发生变性。变性主要表现在核和胞质的电子密度降低；卵黄球相互融合，或卵黄球与膜之间的空隙逐步增大，最终卵黄球从中释放出来，变成裸露体；脂滴数目增多，体积增大；粗面内质网扩大和囊泡变，其上的核糖体脱颗粒等。由此认为，未成熟卵黄细胞在体外培养过程中超微结构的变化可以作为评价培养条件优劣的一个指标。The cultured vitelline cells from adult Schistosoma japonica were observed ultrastructurally. The cells were more sensitive than somatic cells to the changes of in vitro cultured condition, and degenerated in different degrees along with the prolongation of culture. The degeneration was observed earier in mature vitelline cells than in imrnature ones. Changes in the cell structure caused by the degeneration were examined with the following characteristics: the electron density of cells became lower,vitelline globules were fused with each other, the space between vitelline globules and the membrane became larger gradually, vitelline globules were released and uncovered at last. The number and volume of lipid droplets increased. Rough-surfaced endoplasmic reticula enlarged and became vacuolar, and ribosomes left the surface of them. Therefore it was considered that ultrastructural changes of cultured immature vitelline cells could be used to estimate whether the cultured conditions were appropriate.

关 键 词：日本血吸虫 卵黄培养细胞 超微结构 动态变化 

分 类 号：R383.24[医药卫生—医学寄生虫学]