非洲菊未受精胚珠离体培养影响因素研究  

Study on Factors Affecting Unpollinated Ovules in vitro Culture in Gerbera jamesonii Bolus

作　　者：王丽花[1] 瞿素萍[2] 吴学尉[1] 杨秀梅[2] 唐开学[1] 张艺萍[2] 

机构地区：[1]云南省农业科学院花卉研究所.云南省花卉育种重点实验室,云南昆明650205 [2]农业部花卉产品质量监督检验测试中心,云南昆明650205

出　　处：《西南农业学报》2013年第4期1639-1644,共6页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金项目(2009ZC144M);中央补助地方科技基础条件建设项目(财教[2011]210号)

摘　　要：以3个基因型的非洲菊未受精胚珠为试材,研究胚珠发育时期、培养基及供体基因型对非洲菊未受精胚珠离体诱导效果的影响,并以根尖染色体计数为基础对获得的33株再生植株的倍性进行了流式细胞术鉴定。结果表明,胚珠发育时期、培养基及供体基因型均极显著影响愈伤组织和胚状体的诱导频率,且①外轮舌状花完全水平展开、内轮管状花开放前1 d,剥取的胚珠诱导频率较高;②MS中的大量元素和有机物+Heller中的微量元素+1/2铁盐+0.5 mg/L 2,4-D预培养7~10 d有利于启动雌核发育诱导胚状体形成;③经胚状体或愈伤组织2种途径可再生植株,经胚状体途径再生的植株单倍体频率较高。The effects of different ovules development stage, medium and material genotype on the induction of unpollinnted ovulesln d4~ro were studied by using three gonotypic cultivars d Gerbera jamesonllBolus. And ploidy of 33 regeneration plants were identified by flow cy- tomctry based on chromosome counting of root tips. The results showed that ovules development stage, medium and material genotype had significant effects on the callus and embryoids inductivities. And ovules f^om ld before anthesis of the first inner male florels and the full-lev- el open outermoet whorls ligules female flox~,s had higher inductivities. It is beneficial to activate ~nogonic for obtaining the embryoids by preinonhation in the macronutrionts and vitamins of MS and Heller micronutrients and half-strength Fe-EDTA of MS and 0. 5 mg/L 2, 4-D for 7 - 10 d. The ways for embryoids and callus are able to regonemte the plantlet, and haploid induction frequency was higber fzom embry- old pathway.

关 键 词：非洲菊 未受精胚珠 离体培养 

分 类 号：S682.11[农业科学—观赏园艺]