油松花粉黄酮的提取及黄酮酒的制备  

Flavonoid Extraction from Chinese Pine Pollen and Preparation of Flavonoid Wine

作　　者：郭丽梅[1] 王盼盼[1] 杨蒙[1] 武首香[2] 

机构地区：[1]天津科技大学材料科学与化学工程学院,天津300457 [2]天津现代职业技术学院,天津300350

出　　处：《天津科技大学学报》2013年第4期10-13,共4页Journal of Tianjin University of Science & Technology

基　　金：天津市海洋资源与化学重点实验室(天津科技大学)开放基金资助项目(200906)

摘　　要：对油松花粉中的黄酮类物质进行提取、检测，为制作黄酮保健酒提供可靠的工艺路线．采用醇提法对油松花粉中的黄酮进行提取，利用分光光度法以及高效液相色谱法（HPLC）对黄酮的总含量及种类进行了检测．从提取时间、固液比、白酒的酒精体积分数方面考察了最佳的提取条件，确定黄酮的最大提取量，并将之与花粉的浸出实验结果相对比，确定最佳的制作工艺．结果显示，黄酮酒的最佳制作工艺为：采用酒精体积分数为42％的白酒直接浸泡，固液比（g：mL）为1：15，浸泡时间为10-15d，总黄酮的提取量为1．9mg／g．To determine the content and the varieties of flavonoid in Chinese pine pollen so as to better the flavonoid wine making process, extraction and soaking conditions of flavonoid were studied. The total flavonoid content was detected by UV-VIS spectrophotometry and the varieties were studied with HPLC. Time, solid-to-liquid ratio and ethanol content were chosen as the main factors. Through the comparison of extraction process and soaking process, the optimal conditions were determined as follows： the pollen should be directly soaked in 42% ethanol, the solid-to-liquid ratio （g ： mL） was 1 ： 15, and the time was 10 - 15 days. The total flavonoid extracted was 1.9 mg/g.

关 键 词：油松花粉 黄酮 高效液相色谱法 分光光度法 

分 类 号：TQ911[化学工程]