7.2分支H5N1亚型禽流感病毒反向遗传疫苗候选株的构建  

Generation of candidate vaccine virus against the clade 7.2 H5N1 avian influenza variant

作　　者：范俊[1] 李旭勇[1] 王金良[1] 郭晶[1] 邓国华[1] 施建忠[1] 陈化兰[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2013年第9期764-766,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：为应对clade7.2 H5N1亚型禽流感病毒(AIV)持续发生的抗原性改变及其引发的潜在AIV的地方性流行,需要根据流行监测的抗原分析结果挑选合适的供体株制备疫苗候选株。本研究采用反向遗传操作系统,以A/Puerto Rico/8/34(PR8)的内部基因为骨架,以clade7.2 H5N1 AIV A/Chicken/Liao Ning/S4092/2011(CK/LN/S4092)基因组为模板经RT-PCR扩增其HA及NA基因,并对HA基因进行分子修饰,去除与H5亚型AIV致病力有关的HA蛋白裂解位点处的多个连续碱性氨基酸,使其获得低致病性AIV的分子特征(即将-PQIEGRRRKR-突变为-PQRETR-),从而构建出clade7.2 H5N1亚型AIV疫苗候选株rPR8-LN/S4092,为动物免疫试验奠定了基础。To control of the continuing accumulation of antigenic changes within Clade 7.2 highly pathogenic avian influenza viruses （HPAIVs）, and the potential pandemic threat to poultry or even humans, it is necessary to update our vaccine stock according to the antigenic analysis based on surveillance. In this study, a reassortant virus, named rPR8-LN/S4092, was generated using reverse genetics. The virus contained modified HA and NA genes of an epidemic strain A/Chicken/Liao Ning/S4092/2011 in the background of internal genes derived form A/Puerto Rico/8/34 （PR8）. The reassortant virus was attenuated by removal of the multi-basic amino acid motif in the HA gene by mutation and deletion （from PQIEGRRRKR to PQRETR）, which could be served as a candidate vaccine virus.

关 键 词：CLADE 7 2 H5N1流感病毒 反向遗传操作 疫苗候选株 

分 类 号：S852.65[农业科学—基础兽医学]