枸杞体细胞胚发生中DNA代谢动态的立体计量  

Quantitative Stereologic Analysis of Changes in the Metabolic Kinesis of DNA During Somatic Embryogenesis

作　　者：戴若兰[1] 张玮[1] 邢更生[1] 李杉[1] 王亚馥[1] 

机构地区：[1]兰州大学干旱农业生态国家重点实验室,兰州730000

出　　处：《植物学通报》2000年第1期80-83,共4页Chinese Bulletin of Botany

基　　金：国家自然科学基金资助项目!编号:3 9770 3 1 75

摘　　要：本文以宁夏枸杞无菌苗叶片为材料,离体培养,并诱导体细胞胚胎发生。根据细胞形态计量学原理,应用数字图像处理软件计量由光学底片经A/D转换成的数字图像中的DNA大分子,对枸杞体细胞胚发生过程中DNA分子的代谢动态进行量化分析。结果表明:在整个体细胞胚发生过程中DNA代谢呈现动态变化。非胚性细胞与胚性细胞期的量化值分别为 1.82 %和 1.91 %;在二细胞胚、四细胞胚、多细胞胚时期DNA缓慢增长,随着胚性愈伤组织的发育,DNA的含量在梨形胚时期达到高峰;成熟胚的DNA含量虽有所下降,但仍维持较高水平。Using leaves of Lycium barbarum L. as explant to induce somatic embryogenesis. The DNA in somatic embryos of Lycium barbarum L. were analysed using quantitative stereological procedure formed from cell morphometry. The results indicated that the quantity of DNA changes during somatic embryogenesis. The quantitive value of DNA was 1.82% and 1.92% respectively in nonembryonic cells and the embryonic; during bicellular stage, quadracellular stage and multicellular stage, content of DNA increase slowly, and reach the peak at pear_shaped embryonic stage keep high level at mature embryonic stage. We concluded that dynamic changes of DNA has close relationship with embryogenesis and cell reproduction.

关 键 词：枸杞 体细胞胚 DNA合成 立体计量 

分 类 号：Q944.4[生物学—植物学]